Mfg Part Number
Ab01700-1.1
Vector Labs Ab01700-1.1 Anti-E Cadherin [4A2], Mouse IgG1, lambda (200 μg)
Quick Overview
The original mouse IgG1 version of this antibody was raised by immunizing mice with the recombinant maltose binding protein fused to full length human E-Cadherin.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4A2 |
| Synonyms: | Cadherin-1; CD324; Cadherin E; E-Cadherin; epithelial cadherin; CAM 120/80; Uvomorulin |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; IF; FC; WB; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognizes E cadherin which is one of the most important molecules in cell-cell adhesion in epithelial tissues. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. The 4A2 monoclonal antibody recognizes the cytoplasmic domain of E-cadherin. The epitope has been mapped to residues 757–778. |
| Application Notes: | 4A2 antibody has been widely used for identification and analysis of E cadherin. The original mouse IgG1 version was used to detect the intracellular domain of E cadherin in A431 or A431-E-cad-M cells (Nieman et al., 1999; pmid: 10212155). It was also demonstrated that this antibody can detect E cadherin on Western blots (Li et al., 2019; pmid: 31245295). Similarly, 4A2 antibody was also utilized to immunoprecipitate E cadherin from LNCaP and C4-2 cell extracts (Jaggi et al., 2005; pmid: 15695390). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |