Mfg Part Number
Ab01700-10.3
Vector Labs Ab01700-10.3 Anti-E Cadherin [4A2], Human IgG1, Fc Silent™, lambda (200 μg)
Quick Overview
The original mouse IgG1 version of this antibody was raised by immunizing mice with the recombinant maltose binding protein fused to full length human E-Cadherin.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4A2 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Cadherin-1; CD324; Cadherin E; E-Cadherin; epithelial cadherin; CAM 120/80; Uvomorulin |
| Amount: | 200 μg |
| Application Codes Clone: | IHC; IF; FC; WB; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes E cadherin which is one of the most important molecules in cell-cell adhesion in epithelial tissues. It is localized on the surfaces of epithelial cells in regions of cell-cell contact known as adherens junctions. The 4A2 monoclonal antibody recognizes the cytoplasmic domain of E-cadherin. The epitope has been mapped to residues 757–778. |
| Application Notes: | 4A2 antibody has been widely used for identification and analysis of E cadherin. The original mouse IgG1 version was used to detect the intracellular domain of E cadherin in A431 or A431-E-cad-M cells (Nieman et al., 1999; pmid: 10212155). It was also demonstrated that this antibody can detect E cadherin on Western blots (Li et al., 2019; pmid: 31245295). Similarly, 4A2 antibody was also utilized to immunoprecipitate E cadherin from LNCaP and C4-2 cell extracts (Jaggi et al., 2005; pmid: 15695390). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |