Mfg Part Number
Ab01996-23.0
Vector Labs Ab01996-23.0 Anti-PERV [7C4], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by immunization of BALB/c mice with a recombinant protein containing the region from amino acid positions 332 to 615 (Env332–615) of the Env protein of type A PERV.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 7C4 |
| Synonyms: | Porcine Endogenous Retrovirus C; type A PERV Env protein; env |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; Neutralize |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes and binds an epitope between amino acids 517–537 on the p15E component of type A PERV Env protein. |
| Application Notes: | This is a neutralizing antibody againt the porcine endogenous retrovirus. This antibody is unable to pull down the viral particle in an immunoprecipitation assay and has a relatively high IC50 in the neutralization assay which supports the notion that the neutralizing activity of 7C4 is due to the blockage of p15E fusogenic activity after the virus binds to the host cell receptor. Epitope mapping of the antibody was also done using Western blotting and verification of binding sites of the antibody was done by competition ELISA. It was reported that Env332–615 and Env460–539 recombinant proteins exhibited strong inhibition activity for 7C4 mAb binding to the immobilized antigen Env332–615 (Chiang et al., 2007). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |