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Mfg Part Number

Ab02003-1.27

Vector Labs Ab02003-1.27 Anti-Polyglutamine [MW1], Mouse Fab fragment, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised against a 53 amino acid fragment of atrophin-1 containing poly Q (19) and purified from ascites fluid by protein G affinity chromatography.

$517.40

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Description

Product Class: Purified
Clone ID: MW1
Synonyms: PolyQ; HTT; htt; Huntington disease protein; HD protein; huntingtin; Glutamine repeats; Atrophin-1
Amount: 200 μg
Application Codes Clone: WB; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds the extended polyglutamine repeats (GQQQQQQQQQQG) of the mutant huntingtin exon 1. It does not bind the wild type Htt.
Application Notes: This antibody strongly prefers to bind the expanded polyQ repeat forms of the mutant huntingtin and displayed no detectable binding to normal huntingtin in Western blot assay. These results suggested that the polyQ domain can assume different conformations that are distinquishable by mAbs. Immunohistochemistry with wild type (WT) and mutant Htt transgenic mouse (R6) brains shows that different antibodies have different staining patterns. MW1 shows punctate cytoplasmic and neuropil staining in immunohistochemical staining (PMID: 11719267). When MW1 scFv is co-transfected in 293 cells with htt exon 1 containing an expanded polyQ domain, it stimulate htt aggregation and apoptosis (PMID: 11792860). The crystal structure of polyQ bound to MW1, reveals that polyQ adopts an extended, coil-like structure. Consistent with the linear lattice model, multimeric MW1 Fvs bind more tightly to longer than to shorter polyQ tracts and, compared with monomeric Fv, bind expanded polyQ repeats with higher apparent affinities (PMID: 17450152).

Product Class:	Purified
Clone ID:	MW1
Synonyms:	PolyQ; HTT; htt; Huntington disease protein; HD protein; huntingtin; Glutamine repeats; Atrophin-1
Amount:	200 μg
Application Codes Clone:	WB; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds the extended polyglutamine repeats (GQQQQQQQQQQG) of the mutant huntingtin exon 1. It does not bind the wild type Htt.
Application Notes:	This antibody strongly prefers to bind the expanded polyQ repeat forms of the mutant huntingtin and displayed no detectable binding to normal huntingtin in Western blot assay. These results suggested that the polyQ domain can assume different conformations that are distinquishable by mAbs. Immunohistochemistry with wild type (WT) and mutant Htt transgenic mouse (R6) brains shows that different antibodies have different staining patterns. MW1 shows punctate cytoplasmic and neuropil staining in immunohistochemical staining (PMID: 11719267). When MW1 scFv is co-transfected in 293 cells with htt exon 1 containing an expanded polyQ domain, it stimulate htt aggregation and apoptosis (PMID: 11792860). The crystal structure of polyQ bound to MW1, reveals that polyQ adopts an extended, coil-like structure. Consistent with the linear lattice model, multimeric MW1 Fvs bind more tightly to longer than to shorter polyQ tracts and, compared with monomeric Fv, bind expanded polyQ repeats with higher apparent affinities (PMID: 17450152).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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