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Mfg Part Number

Ab02004-1.27

Vector Labs Ab02004-1.27 Anti-Polyglutamine [1C2], Mouse Fab fragment, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised against the full length TATA binding protein (TBP) containing a poly Q sequnce with 38 repeats of glutamine and collected from the ascites fluid of the mice.

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Description

Product Class: Purified
Clone ID: 1C2
Synonyms: PolyQ; Glutamine repeats; TATA binding protein; TBP
Amount: 200 μg
Application Codes Clone: WB; ELISA; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody bind the extended polyglutamine repeats found in the TATA binding protein. It is reported that the affinity of this mAb is proportional to the length of the polyglutamine chain and that this antibody recognizes polyQ stretches with 11, 22 and 41 amino acids.
Application Notes: This antibody recognizes recombinant and endogenous HeLa cell TBP in Western blots. Epitope mapping of the antibody was done using ELISA by incubating the mAbs with synthetic overlapping peptides having 150 amino acids from the N-terminal of the HeLa cell TBP. Thia antibody immunoprecipitates natibe TFIID from phosphocellulose 1 M KCl fractions indicating that the N-terminal region of TBP is not blocked by TBP binding proteins. It is reported that mAb 1C2 selectively inhibited in vitro transcription from TATA-containing, but not from TATA-less promoters, irrespective of whether they were transcribed by Pol II or Pol III (PMID:7510635). This antibody is reported to successfully recognize the polyglutamine chains of mutated alleles of protein from several human neurodegenerative diseases caused by a CAG repeat expansion, like Huntington's disease and spinocerebellar ataxia type 2, 3 and 7 (PMID: 7477379). It is reported that this antibody recognises the linear and extended conformations of polyglutamine (PMID: 23777629).

Product Class:	Purified
Clone ID:	1C2
Synonyms:	PolyQ; Glutamine repeats; TATA binding protein; TBP
Amount:	200 μg
Application Codes Clone:	WB; ELISA; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody bind the extended polyglutamine repeats found in the TATA binding protein. It is reported that the affinity of this mAb is proportional to the length of the polyglutamine chain and that this antibody recognizes polyQ stretches with 11, 22 and 41 amino acids.
Application Notes:	This antibody recognizes recombinant and endogenous HeLa cell TBP in Western blots. Epitope mapping of the antibody was done using ELISA by incubating the mAbs with synthetic overlapping peptides having 150 amino acids from the N-terminal of the HeLa cell TBP. Thia antibody immunoprecipitates natibe TFIID from phosphocellulose 1 M KCl fractions indicating that the N-terminal region of TBP is not blocked by TBP binding proteins. It is reported that mAb 1C2 selectively inhibited in vitro transcription from TATA-containing, but not from TATA-less promoters, irrespective of whether they were transcribed by Pol II or Pol III (PMID:7510635). This antibody is reported to successfully recognize the polyglutamine chains of mutated alleles of protein from several human neurodegenerative diseases caused by a CAG repeat expansion, like Huntington's disease and spinocerebellar ataxia type 2, 3 and 7 (PMID: 7477379). It is reported that this antibody recognises the linear and extended conformations of polyglutamine (PMID: 23777629).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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