Mfg Part Number
Ab02017-10.160
Vector Labs Ab02017-10.160 Anti-Spike protein (RBD) [Sb#68], Human IgG1-Fc Fusion, Fc Silent™, (200 μg)
Quick Overview
This clone was originally isolated in a form of a synthetic nanobody (sybody) via a 'target swap' selection procedure against RBD-vYFP using ribosomal display and against RBD-Fc fusion during phage display rounds.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | Sb#68 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | SARS CoV 2 S glycoprotein; Sb68; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV; SARS-CoV2 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; inhibition; grating-coupled interferometry; therapeutics |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes and binds the SARS CoV 2 C-terminal receptor binding domain (RBD) located in the extracellular portion of the spike protein. |
| Application Notes: | This antibody is recommended for detection of SARS CoV 2 or 2019-nCoV. Sb#68 is a strong binder and binds the 2019-nCoV ectodomain with an affinity of KD ≈ 37 nM (Walter et al., 2020). The initial characterization and binding activity of this antibody to SARS CoV2 spike protein was confirmed with ELISA (Walter et al., 2020). This antibody is a mild inhibitor for interaction between hACE2 and RBD of SARS CoV2. This clone binds to a non-overlapping epitope relative to S#15 (Ab02013) what can be used in develoepment of anti-Covid-19 therapuetics granting together increased avidity to the spike protein (Walter et al., 2020). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |