Mfg Part Number
Ab02019-1.1
Vector Labs Ab02019-1.1 Anti-Spike Protein (RBD) [CV30], Mouse IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by isolating B-cells from the peripheral blood mononuclear cells (PBMCs) of SARS CoV2 infected patients, after the serum neutralizing activity was confirmed.
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| Product Class: | Purified |
|---|---|
| Clone ID: | CV30 |
| Synonyms: | Receptor Binding Domain; SARS-CoV2; SARS Coronavirus 2; SARS-CoV-2; SARS CoV 2; 2019-nCoV; SARS Coronavirus 2 receptor binding domain; SARS CoV2 RBD; Severe acute respiratory syndrome coronavirus 2; Spike protein; S protein; SARS-CoV 2 S protein; S glycoprotein; E2; Peplomer protein |
| Amount: | 200 μg |
| Application Codes Clone: | Neutralize; ELISA; FC; Therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original human IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes and binds the receptor binding domain (RBD) encoded by residues 319-591 of the SARS CoV 2 spike protein. CV30 binds the SARS CoV 2 RBD with a high affinity of 3.6nM and completely blocks the interaction between the RBD and ACE2 domain. |
| Application Notes: | This antibody is a potent 2019-nCoV neutralizing antibody and highly recommended for diagnosis and/or treatment of SARS CoV 2 or 2019-nCoV. CV30 binds SARS CoV 2 RBD with a very high affinity and achieves 100% neutralization (IC50=0.03µg/ml). ELISA was used to confirm the binding of SARS CoV 2 positive serum samples to the entire ectodomain and the receptor binding domain of the spike protein. Specific binding of the mAb to SARS CoV 2 to receptor binding domain was confirmed with biolayer interferometry. Phycoerythrin labelled mAb was used to stain 293E cells transfected with wild type SARS CoV 2 and were analysed by flow cytometry (Seydoux et al., 2020). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |