Vector Labs Ab02025-23.0 Anti-IL-6 [mAB1339 (OP-R003-1)], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: mAB1339 (OP-R003-1)
Synonyms: IL6; IFNB2; BSF-2; CDF; Interleukin-6; IL-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
Amount: 200 μg
Application Codes Clone: FC; ELISA; Blocking; Therapeutic
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes and binds the interleukin-6 at amino acid residues that are crucial for binding to human IL-6R. The binding of this antibody blocks or inhibits the binding between human IL6 and human IL-6R.
Application Notes: This high affinity, fully human antagonistic monoclonal antibody mAb 1339 or OP-R003-1 was structurally derived from elsilimomab (B-E8, a murine anti-IL-6 mAb). In vitro studies demonstrated that mAb 1339 shares similar biological properties, including affinity and epitope specificity with its “parent” murine antibody. mAb 1339 detected human and monkey IL-6 but not murine or rat IL-6. No binding was observed with other IL-6 superfamily members. The binding affinity of this antibody to IL-6 was tested using ELISA. This fully human mAb inhibited the binding of IL-6 to its receptor as evidenced by flow cytometry using U266 cells and also inhibited IL-6 induced cell proliferation in murine B9, human TF1 or U266 cells (Vermot-Desroches et al., 2007). mAb 1339 inhibited the growth of multiple myeloma in presence of BMSC vitro, associated with inhibition of phosphorylation of STAT3, ERK1/2 and Akt and synergistically enhanced the cytotoxic activity of dexamethasone as well as bortezomib, lenalidomide, and perifosine. It also significantly enhanced growth inhibitory effects of dexamethasone in vivo in a SCID-hu mouse model of multiple myeloma (PMID: 19934301)

Product Class:	Purified
Clone ID:	mAB1339 (OP-R003-1)
Synonyms:	IL6; IFNB2; BSF-2; CDF; Interleukin-6; IL-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; IFN-beta-2
Amount:	200 μg
Application Codes Clone:	FC; ELISA; Blocking; Therapeutic
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes and binds the interleukin-6 at amino acid residues that are crucial for binding to human IL-6R. The binding of this antibody blocks or inhibits the binding between human IL6 and human IL-6R.
Application Notes:	This high affinity, fully human antagonistic monoclonal antibody mAb 1339 or OP-R003-1 was structurally derived from elsilimomab (B-E8, a murine anti-IL-6 mAb). In vitro studies demonstrated that mAb 1339 shares similar biological properties, including affinity and epitope specificity with its “parent” murine antibody. mAb 1339 detected human and monkey IL-6 but not murine or rat IL-6. No binding was observed with other IL-6 superfamily members. The binding affinity of this antibody to IL-6 was tested using ELISA. This fully human mAb inhibited the binding of IL-6 to its receptor as evidenced by flow cytometry using U266 cells and also inhibited IL-6 induced cell proliferation in murine B9, human TF1 or U266 cells (Vermot-Desroches et al., 2007). mAb 1339 inhibited the growth of multiple myeloma in presence of BMSC vitro, associated with inhibition of phosphorylation of STAT3, ERK1/2 and Akt and synergistically enhanced the cytotoxic activity of dexamethasone as well as bortezomib, lenalidomide, and perifosine. It also significantly enhanced growth inhibitory effects of dexamethasone in vivo in a SCID-hu mouse model of multiple myeloma (PMID: 19934301)

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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