Mfg Part Number
Ab02026-23.0
Vector Labs Ab02026-23.0 Anti-IL-6 [68F2], Rabbit IgG, lambda (200 μg)
Quick Overview
The original antibody was generated by immunizations of Llama glama with recombinant IL-6 followed by construction of phage display library, selection, screeing and purification.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 68F2 |
| Synonyms: | IL6; IFNB2; BSF-2; CDF; Interleukin-6; IL-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; IFN-beta-2 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody recognizes and binds interleukin-6 near the binding site I near the N-terminal part of α-helix 1 and the C-terminal part of α-helix 4 of the four-helix bundle, as well as the long loop between α-helices 1 and 2. This binding interface of 68F2 to a major extent overlaps with site I recognized by IL-6R. |
| Application Notes: | This antibody is recommended for identification of IL-6 in the sample. The ability of this antibody to compete with biotinylated IL-6 for binding to captured IL-6R was investigated using competitive ELISA. The in-vitro potency of the antibody was determined using a cell-based neutralizing bioassay using the B9 and 7TD1 cell line, which require IL-6 for survival and proliferation and respond to very low concentration of IL-6 (PMID: 27129274). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |