Mfg Part Number
Ab02028-10.0
Vector Labs Ab02028-10.0 Anti-LMP1 [HLEAFab], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated from a human Fab phage library after panning against HNE2 cell line and HNE2/LMP1 cell line, as well as on immobilized LMP1 extracellular domains. The phages with specific binding for extracellular domains were selected after seven rounds of panning.
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| Product Class: | Purified |
|---|---|
| Clone ID: | HLEAFab |
| Synonyms: | LMP-1; p63; Latent membrane protein 1; protein p63 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; IF; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds to the LMP1 extracellular domain in native confirmation. |
| Application Notes: | This antibody binds to the extracellular domain of LMP1, which was confirmed with ELISA. The ability of the expressed HLEAFab to specifically bind to native LMP1 on the HNE2/LMP1 cell surface was further confirmed by Cell ELISA. The binding was also confirmed using flow cytometry and immunofluorescence staining. An immunoconjugate fof HLEAFab and mitomycin C (HLEAFab-MMC) inhibits proliferation and induced apoptosis in nasopharyngeal carcinoma (NPC) cell line HNE2/LMP1 in vitro and is also found to show inhibition of growth of NPC xenografts in nude mice (PMID: 22169768). HLEAFab works synergistically with mitomycin C to inhibit growth of nasopharyngeal carcinoma growth in vivo through inducing apoptosis and downregulating vascular endothelial growth factor (VEGF). Immunohistochemical staining showed that VEGF expression in NPC xenografts was significantly inhibited in the combination group compared to the Fab (PMID: 22408448). HLEAFab is recommended to be used to detect LMP1 expression in NPC tissues using immunohistochemical staining. Positive staining of LMP1 was observed in the membrane and cytoplasm of NPC cells (PMID: 23760364). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |