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Mfg Part Number

Ab02045-10.3

Vector Labs Ab02045-10.3 Anti-Migis-alpha [29C11], Human IgG1, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing BALB/c mice with a mouse/human chimeric migis-αL-containing protein.

$517.40

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Description

Product Class: Purified
Clone ID: 29C11
Heavy Chain Modification: Fc Silent™
Synonyms: Migis-α; mIgA isotype-specific extracellular peptide; extracellular membrane proximal domain of mIgA α chain
Amount: 200 μg
Application Codes Clone: ELISA; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds the extracellular peptide called the Migis-α consisting of 26-32 amino acids towards the N-terminal of the human membrane bound IgA, when the lipid rafts are disrupted.
Application Notes: Membrane bound IgA is associated with Igα/Igβ as the B cell receptor (BCR) complex on mIgA-expressing B cells. The α chain of mIgA (mα) contains an extracellular domain that is reported to be an antigenic site suitable for isotype-specific targeting of mIgA-expressing B cells by antibodies. The mAb binds strongly to synthetic peptides of migis-α and to various recombinant proteins containing migis-α as revealed by ELISA. Flow cytometric analysis with B cells suggested that the mAb did not bind strongly to mIgA and required a higher concentration for detection. Immunoprecipitation analysis of these mAbs indicated that mIgA could only be pulled down by 29C11 when mIgA-expressing B cells were solubilized by strong detergents, such as sodium dodecyl sulfate (SDS) or when lipid rafts are disrupted (PMID: 21723611).

Product Class:	Purified
Clone ID:	29C11
Heavy Chain Modification:	Fc Silent™
Synonyms:	Migis-α; mIgA isotype-specific extracellular peptide; extracellular membrane proximal domain of mIgA α chain
Amount:	200 μg
Application Codes Clone:	ELISA; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds the extracellular peptide called the Migis-α consisting of 26-32 amino acids towards the N-terminal of the human membrane bound IgA, when the lipid rafts are disrupted.
Application Notes:	Membrane bound IgA is associated with Igα/Igβ as the B cell receptor (BCR) complex on mIgA-expressing B cells. The α chain of mIgA (mα) contains an extracellular domain that is reported to be an antigenic site suitable for isotype-specific targeting of mIgA-expressing B cells by antibodies. The mAb binds strongly to synthetic peptides of migis-α and to various recombinant proteins containing migis-α as revealed by ELISA. Flow cytometric analysis with B cells suggested that the mAb did not bind strongly to mIgA and required a higher concentration for detection. Immunoprecipitation analysis of these mAbs indicated that mIgA could only be pulled down by 29C11 when mIgA-expressing B cells were solubilized by strong detergents, such as sodium dodecyl sulfate (SDS) or when lipid rafts are disrupted (PMID: 21723611).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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