Vector Labs Ab02051-23.0 Anti-HIV-1 GP120 [0.5B], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 0.5B
Synonyms: Gp120; Human immunodeficiency virus 1; Envelope glycoprotein gp120
Amount: 200 μg
Application Codes Clone: NTRL; FC; IP; WB; ELISA; In vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds an epitope between amino acid residues 308 to 331 located in the V3 loop of the HIV-1 IIIB family of viruses.
Application Notes: 0.5 Beta is a neutralizing antibody that binds the external envelope glycoprotein gp120 of the HIV-1. The binding specificity of this antibody was checked using flow cytometry. The epitope to which 0.5 beta binds has homologous sequences with inter-alpha-trypsin inhibitor (Koito et al., 1989; PMID:2484964). This antibody in conjugation with immunotoxins like ricin A chain (RAC) or pseudomonas exotoxin (PE) was used to selectively inhibit proliferation and kill HIV infected cells. The humanized version of 0.5 beta retained both the binding affinity and neutralizing activity compared to its parent murine antibody and chimeric antibody. Furthermore this antibody was used for an ELISA on a synthetic 20 amino acid peptide (308-327) based on the sequence of the RP135 region of gp120 from HIV-IIIB (Maeda et al., 1991; PMID: 1873503). This antibody was used for a western blot on HTLV-IIIB antigens (Matsushita et al., 1988; PMID:2452899), and on Purified HTLV-IIIB (Skinner et al., 1988; PMID:2456088). The NMR structure of this antibody was determined (Tugarinov et al., 2000; PMID:10801487). The ability of this antibody to neutralize a cell-free HIV infection of H9 cells was assayed. It was indeed able to prevent this infection (Matsushita et al., 1988; PMID:2452899). This antibody was also able to neutralize the HIV infection of AA-5 cells (Skinner et al., 1988; PMID:2456088). This antibody was able to prevent HIV infection in chimpanzees in vivo (Emini et al., 1992; PMID:1741059).

Product Class:	Purified
Clone ID:	0.5B
Synonyms:	Gp120; Human immunodeficiency virus 1; Envelope glycoprotein gp120
Amount:	200 μg
Application Codes Clone:	NTRL; FC; IP; WB; ELISA; In vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds an epitope between amino acid residues 308 to 331 located in the V3 loop of the HIV-1 IIIB family of viruses.
Application Notes:	0.5 Beta is a neutralizing antibody that binds the external envelope glycoprotein gp120 of the HIV-1. The binding specificity of this antibody was checked using flow cytometry. The epitope to which 0.5 beta binds has homologous sequences with inter-alpha-trypsin inhibitor (Koito et al., 1989; PMID:2484964). This antibody in conjugation with immunotoxins like ricin A chain (RAC) or pseudomonas exotoxin (PE) was used to selectively inhibit proliferation and kill HIV infected cells. The humanized version of 0.5 beta retained both the binding affinity and neutralizing activity compared to its parent murine antibody and chimeric antibody. Furthermore this antibody was used for an ELISA on a synthetic 20 amino acid peptide (308-327) based on the sequence of the RP135 region of gp120 from HIV-IIIB (Maeda et al., 1991; PMID: 1873503). This antibody was used for a western blot on HTLV-IIIB antigens (Matsushita et al., 1988; PMID:2452899), and on Purified HTLV-IIIB (Skinner et al., 1988; PMID:2456088). The NMR structure of this antibody was determined (Tugarinov et al., 2000; PMID:10801487). The ability of this antibody to neutralize a cell-free HIV infection of H9 cells was assayed. It was indeed able to prevent this infection (Matsushita et al., 1988; PMID:2452899). This antibody was also able to neutralize the HIV infection of AA-5 cells (Skinner et al., 1988; PMID:2456088). This antibody was able to prevent HIV infection in chimpanzees in vivo (Emini et al., 1992; PMID:1741059).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews