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Mfg Part Number

Ab02052-10.0

Vector Labs Ab02052-10.0 Anti-alpha-Toxin [3A4D10], Human IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The orignal antibody was generated by immunizing BALB/c mice with an immunogenic toxoid prepared by incubating the alpha toxin with formaldehyde.

$517.40

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Description

Product Class: Purified
Clone ID: 3A4D10
Synonyms: PLC; Phospolipase C; Zn-metallo phospholipase C; Alpha-toxin; Hemolysin; Lecithinase; Phosphatidylcholine cholinephosphohydrolase; α-Toxin
Amount: 200 μg
Application Codes Clone: ELISA; WB; NTRL
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds the alpha toxin of Clostridium perfringens. It specifically recognizes and binds the epitope 'ARGFAK' of the toxin.
Application Notes: Alpha toxin is an exotoxin, a type of bacterial haemolysin that attack blood cell membranes and cause cell rupture. Alpha toxin is an essential virulance factor in gas gangrene and causes myonecrosis in infected tissues. ELISA was used to identify the specific clones binding to alpha-toxin and epitope mapping of the antibody. 3A4D10 can even recognize denatrued toxin in a western blot assay. This antibody is capable of neutralizing the toxin in vitro and in vivo assays (PMID: 1718874). Only immunization with amino acids 247-370 of the Clostridium perfringens alpha toxin induced protection against the lethal effects of the toxin. The toxin plays a major role in pathogenesis of gas gangrene (PMID: 825650). Humanized version derived from its murine parent showed higher binding affinity (PMID: 7716162).

Product Class:	Purified
Clone ID:	3A4D10
Synonyms:	PLC; Phospolipase C; Zn-metallo phospholipase C; Alpha-toxin; Hemolysin; Lecithinase; Phosphatidylcholine cholinephosphohydrolase; α-Toxin
Amount:	200 μg
Application Codes Clone:	ELISA; WB; NTRL
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds the alpha toxin of Clostridium perfringens. It specifically recognizes and binds the epitope 'ARGFAK' of the toxin.
Application Notes:	Alpha toxin is an exotoxin, a type of bacterial haemolysin that attack blood cell membranes and cause cell rupture. Alpha toxin is an essential virulance factor in gas gangrene and causes myonecrosis in infected tissues. ELISA was used to identify the specific clones binding to alpha-toxin and epitope mapping of the antibody. 3A4D10 can even recognize denatrued toxin in a western blot assay. This antibody is capable of neutralizing the toxin in vitro and in vivo assays (PMID: 1718874). Only immunization with amino acids 247-370 of the Clostridium perfringens alpha toxin induced protection against the lethal effects of the toxin. The toxin plays a major role in pathogenesis of gas gangrene (PMID: 825650). Humanized version derived from its murine parent showed higher binding affinity (PMID: 7716162).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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