Vector Labs Ab02054-10.0 Anti-CD18 [H52], Human IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: H52
Synonyms: ITGB2; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; β2-subunit of leukocyte integrins
Amount: 200 μg
Application Codes Clone: Block; Inhibit; FC; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds disulfide-bonded loop (C386and C400) in the beta-2 integrin subunit. It is reported that the epitope of H52 is masked in the integrin Mac-1 and p150,95.
Application Notes: This antibody is successfully capable of inhibiting T cell, natural killer cells and complement receptor type 3 functions. Binding specificity of this antibody was confirmed with immunoprecipitation (PMID: 2580022). H52 mAb blocked 8E5-PHA-blast fusion in a dose-dependent manner with complete inhibition observed at concentrations above 3 μg/ml. Cell fusion (syncytium formation) is a major cytopathic effect of infection by human immunodeficiency virus (HIV). To determine the effect of the mAb on syncytium formation the LFA-1 expression of 8E5 cells and PHA-blasts was checked by flow cytometry. This mAb inhibited HIV-induced syncytium formation, however it did not block binding of gp120 to CD4 (PMID: 2543075). This mAb cross reacts with procine LFA-1 and profoundly inhibited mitogenic and allogenic stimulation of porcine T lymphocytes as well as cytotoxic T cell and natural killer (NK) cell function (PMID: 2481234). The expression of H52 epitope on beta-2 subunit isdependent on its interation with different alpha subunits of the leukocyte integrin LFA-1, Mac-1 and p150,95 and presence of calcium ions. The expression of the H52 epitope was examined by immunoprecipitation (PMID: 9808198).

Product Class:	Purified
Clone ID:	H52
Synonyms:	ITGB2; MFI7; Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta; Complement receptor C3 subunit beta; β2-subunit of leukocyte integrins
Amount:	200 μg
Application Codes Clone:	Block; Inhibit; FC; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds disulfide-bonded loop (C386and C400) in the beta-2 integrin subunit. It is reported that the epitope of H52 is masked in the integrin Mac-1 and p150,95.
Application Notes:	This antibody is successfully capable of inhibiting T cell, natural killer cells and complement receptor type 3 functions. Binding specificity of this antibody was confirmed with immunoprecipitation (PMID: 2580022). H52 mAb blocked 8E5-PHA-blast fusion in a dose-dependent manner with complete inhibition observed at concentrations above 3 μg/ml. Cell fusion (syncytium formation) is a major cytopathic effect of infection by human immunodeficiency virus (HIV). To determine the effect of the mAb on syncytium formation the LFA-1 expression of 8E5 cells and PHA-blasts was checked by flow cytometry. This mAb inhibited HIV-induced syncytium formation, however it did not block binding of gp120 to CD4 (PMID: 2543075). This mAb cross reacts with procine LFA-1 and profoundly inhibited mitogenic and allogenic stimulation of porcine T lymphocytes as well as cytotoxic T cell and natural killer (NK) cell function (PMID: 2481234). The expression of H52 epitope on beta-2 subunit isdependent on its interation with different alpha subunits of the leukocyte integrin LFA-1, Mac-1 and p150,95 and presence of calcium ions. The expression of the H52 epitope was examined by immunoprecipitation (PMID: 9808198).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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