Vector Labs Ab02057-3.0 Anti-Spike protein [EY6A], Mouse IgG2b, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: EY6A
Synonyms: SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
Amount: 200 μg
Application Codes Clone: NTRL; ELISA; IF; Surface Plasmon Resonance; therapeutic
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds tightly (KD of 2 nM) to the receptor binding domain (RBD) the of the SARS-CoV-2. It also cross reacts with SARS-CoV-1. This antibody recognizes a highly conserved epitope on away from the ACE2 receptor binding domain.
Application Notes: EY6A binds S1 domain of SARS-CoV-2 and also cross reacts with SARS-CoV-1. The binding was confirmed by ELISA. Further confirmation of binding of EY6A to SARS-CoV-2 infected cells was done by immunofluorescence. Surface plasmon resonance (SPR) measurements for EY6A Fab showed high affinity binding to immobilised SARS-CoV-2 RBD (KD = 2 nM). EY6A did not completely block binding of ACE2 to RBD, but was successful in neutralizing SARS-CoV-2 infection in Vero E6 cells (Zhou et al., 2020).

Product Class:	Purified
Clone ID:	EY6A
Synonyms:	SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
Amount:	200 μg
Application Codes Clone:	NTRL; ELISA; IF; Surface Plasmon Resonance; therapeutic
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds tightly (KD of 2 nM) to the receptor binding domain (RBD) the of the SARS-CoV-2. It also cross reacts with SARS-CoV-1. This antibody recognizes a highly conserved epitope on away from the ACE2 receptor binding domain.
Application Notes:	EY6A binds S1 domain of SARS-CoV-2 and also cross reacts with SARS-CoV-1. The binding was confirmed by ELISA. Further confirmation of binding of EY6A to SARS-CoV-2 infected cells was done by immunofluorescence. Surface plasmon resonance (SPR) measurements for EY6A Fab showed high affinity binding to immobilised SARS-CoV-2 RBD (KD = 2 nM). EY6A did not completely block binding of ACE2 to RBD, but was successful in neutralizing SARS-CoV-2 infection in Vero E6 cells (Zhou et al., 2020).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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