Vector Labs Ab02062-15.159 Anti-Spike protein [Sb23], Human IgM-Fc Fusion, (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: Sb23
Synonyms: SARS CoV 2 S glycoprotein; Sb23; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
Amount: 100 μg
Application Codes Clone: NTRL; ELISA; Biolayer Interferometry
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is capable of binding both recombinant RBD as well as to the pre-fusion spike glycoprotein with high affinity. Sb23 binds an epitope in the ACE2 binding site on the RBD in both its ‘up’ and ‘down’ conformation and thereby effectively blocks ACE2 binding.
Application Notes: Sb23 binds RBD with a very high affinity and neutralizes pseudovirus with an IC50 of 0.6 µg/ml. Sb23 and ACE2 compete for the same or overlapping binding sites on SARS-CoV-2-RBD. ELISA was used to screen the libraries to identify specific RBD binders. It is also reported that since Sb23 can efficiently replace ACE2 from a preformed complex, it has a higher affinity for RBD than ACE2. Biolayer interferometry assay was used to kinetically characterize the interaction of sybodies with RBD and spike (Custódio et al., 2020).

Product Class:	Purified
Clone ID:	Sb23
Synonyms:	SARS CoV 2 S glycoprotein; Sb23; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
Amount:	100 μg
Application Codes Clone:	NTRL; ELISA; Biolayer Interferometry
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is capable of binding both recombinant RBD as well as to the pre-fusion spike glycoprotein with high affinity. Sb23 binds an epitope in the ACE2 binding site on the RBD in both its ‘up’ and ‘down’ conformation and thereby effectively blocks ACE2 binding.
Application Notes:	Sb23 binds RBD with a very high affinity and neutralizes pseudovirus with an IC50 of 0.6 µg/ml. Sb23 and ACE2 compete for the same or overlapping binding sites on SARS-CoV-2-RBD. ELISA was used to screen the libraries to identify specific RBD binders. It is also reported that since Sb23 can efficiently replace ACE2 from a preformed complex, it has a higher affinity for RBD than ACE2. Biolayer interferometry assay was used to kinetically characterize the interaction of sybodies with RBD and spike (Custódio et al., 2020).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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