Vector Labs Ab02065-23.0 Anti-Lysozyme [D1.3], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: D1.3
Synonyms: HEL; Hen Egg white Lysozyme; Lysozyme C; 1,4-beta-N-acetylmuramidase C; Allergen Gal d IV; Allergen: Gal d 4
Amount: 200 μg
Application Codes Clone: ELISA; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody specifically binds hen egg-white lysozyme.
Application Notes: Lysozyme is an enzyme that degrades polysacchardies of various types of bacterial cell walls. It is observed that lysozyme bound D1.3 still retains its enzymatic activity. This is attributed to the fact that D1.3 binds lysozyme at a site remote from the catalytic site and that there is no significant conformational change caused by the binding (PMID: 3614217). The three dimension structure of a complex between the Fab from antibody D1.3 and its antigen, HEL, has been determined at 6 and at 2.8 a resolution (PMID: 2426778, 3965976). Sixteen residues of lysozyme make contact with 17 residues of the antibody. The antigenic determinant is topographic including lysozyme residues 18, 19, 21–25, 27, 116–121, 124, and 129. All six complementarity determining regions of the antibody contact the antigen, and in addition, two residues from “framework” segments of V H and V L (Amit et al., 1986; PMID: 2441250). D1.3 binds native lysozyme in an ELISA (PMID: 2448386). Humanized version of this antibody is also available (PMID: 1560463, 2451287). Flow cytometry was used to find the fine difference in affinity of D1.3 (scFv) and mutant M3 clone expressed in a yeast surface display (PMID:10662486).

Product Class:	Purified
Clone ID:	D1.3
Synonyms:	HEL; Hen Egg white Lysozyme; Lysozyme C; 1,4-beta-N-acetylmuramidase C; Allergen Gal d IV; Allergen: Gal d 4
Amount:	200 μg
Application Codes Clone:	ELISA; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody specifically binds hen egg-white lysozyme.
Application Notes:	Lysozyme is an enzyme that degrades polysacchardies of various types of bacterial cell walls. It is observed that lysozyme bound D1.3 still retains its enzymatic activity. This is attributed to the fact that D1.3 binds lysozyme at a site remote from the catalytic site and that there is no significant conformational change caused by the binding (PMID: 3614217). The three dimension structure of a complex between the Fab from antibody D1.3 and its antigen, HEL, has been determined at 6 and at 2.8 a resolution (PMID: 2426778, 3965976). Sixteen residues of lysozyme make contact with 17 residues of the antibody. The antigenic determinant is topographic including lysozyme residues 18, 19, 21–25, 27, 116–121, 124, and 129. All six complementarity determining regions of the antibody contact the antigen, and in addition, two residues from “framework” segments of V H and V L (Amit et al., 1986; PMID: 2441250). D1.3 binds native lysozyme in an ELISA (PMID: 2448386). Humanized version of this antibody is also available (PMID: 1560463, 2451287). Flow cytometry was used to find the fine difference in affinity of D1.3 (scFv) and mutant M3 clone expressed in a yeast surface display (PMID:10662486).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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