Mfg Part Number
Ab02066-10.3
Vector Labs Ab02066-10.3 Anti-HCMV gH [HCMV16], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunization of mice with purified HCMV strain AD169.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | HCMV16 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | gH; Envelope glycoprotein H; gpUL75; Human Cytomegalovirus; HCMV; CMV |
| Amount: | 200 μg |
| Application Codes Clone: | IP; IF; NTRL; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds gpUL75 (gH) of the human cytomegalovirus, which is an 86-kDa glycoprotein. The heterodimer glycoprotein H-glycoprotein L is required for the fusion of viral and plasma membranes leading to virus entry into the host cell. It is though to bind a conformational epitope on the gH, and its bind is destroyed if the protein is denatured. |
| Application Notes: | This antibody successfully precipitates glycoprotein H from both HCMV infected cell lysates and HCMV-gH-VAC-infected cell lysates. Cellular localization of HCMV-gH studied by immunofluorescence on recombinant vaccinia virus and HCMV-infected cells using HCMV-16. HCMV-16 strongly neutralizes HCMV infectivity in vitro (PMID: 2831402). The reshaped humanized antibody recognized cells infected with a variety of virus strains and neutralized clinical isolates of CMV as efficiently as laboratory strains in a conventional plaque reduction assay (PMID: 9207350). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |