Mfg Part Number
Ab02071-3.0
Vector Labs Ab02071-3.0 Anti-CD32 [IV.3], Mouse IgG2b, kappa (200 μg)
Quick Overview
The original antibody was generated against the FcR of K562 cells.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | IV.3 |
| Synonyms: | CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a |
| Amount: | 200 μg |
| Application Codes Clone: | IP; Inhibit; IF; Block; ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody recognizes and binds CD32a. |
| Application Notes: | This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID: 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic “FCGR2A” mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |