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Mfg Part Number

Ab02073-10.0

Vector Labs Ab02073-10.0 Anti-CD32 [MDE-9], Human IgG1, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund’s adjuvant.

$517.40

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Description

Product Class: Purified
Clone ID: MDE-9
Synonyms: CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; FcgammaRIIa-H131; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a
Amount: 200 μg
Application Codes Clone: FC; IHC; ELISA; IF; Block
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a and, more particularly, the FcγRIIa-H131 allotype.
Application Notes: MDE-9 was able to detect FcgammaRIIa-H131 in both flow cytometry and immunohistochemistry. MDE-9 bound FcgRIIa-H131 on cell lines expressing FcgRIIaH131, as well as on peripheral blood monocytic cells and PMN in immunofluorescence assays. MDE-9 was used to develop an FcgammaRIIa allotyping method based on flow cytometry. The results of flow cytometric FcgammaRIIa allotyping correlated completely with PCR genotyping. This novel allotyping assay can facilitate the screening of patients in a routine diagnostic setting. A combination of MDE-9 and mAb 41H16 can be used in FcgammaRIIa-H/H131 homozygous individuals to detect FcgammaRIIa and FcgammaRIIb surface expression on monocytes (PMID: 15604023).

Product Class:	Purified
Clone ID:	MDE-9
Synonyms:	CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII; FcRII-a; FCGR2A; FcgammaRIIa-H131; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc region receptor II-a
Amount:	200 μg
Application Codes Clone:	FC; IHC; ELISA; IF; Block
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a and, more particularly, the FcγRIIa-H131 allotype.
Application Notes:	MDE-9 was able to detect FcgammaRIIa-H131 in both flow cytometry and immunohistochemistry. MDE-9 bound FcgRIIa-H131 on cell lines expressing FcgRIIaH131, as well as on peripheral blood monocytic cells and PMN in immunofluorescence assays. MDE-9 was used to develop an FcgammaRIIa allotyping method based on flow cytometry. The results of flow cytometric FcgammaRIIa allotyping correlated completely with PCR genotyping. This novel allotyping assay can facilitate the screening of patients in a routine diagnostic setting. A combination of MDE-9 and mAb 41H16 can be used in FcgammaRIIa-H/H131 homozygous individuals to detect FcgammaRIIa and FcgammaRIIb surface expression on monocytes (PMID: 15604023).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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