Mfg Part Number
Ab02074-23.159
Vector Labs Ab02074-23.159 Anti-Gliadin [VHH26], Rabbit IgG-Fc Fusion, kappa (100 μg)
Quick Overview
The original antibody was prepared by immunization of Llama glama with whole gliadin.
$692.90
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | VHH26 |
| Synonyms: | Alpha-gliadin; α-gliadin; Celiac Allergen; Coeliac Allergen |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody specifically recognizes and binds and epitope unique to wheat alpha gliadins. |
| Application Notes: | This antibody specifically binds an epitope unique for wheat alpha gliadins which is not present in gamma or omega gliadins. It does not cross reat with rye or barley gliadins. VHH26 can detect gliadins in denaturing conditions and it retains its binding capacity in presence of 15% ethanol, 0.5% 2-mercaptoethanol, and 0.5 M guanidinium chloride. Capture ELISA using VHH26 was able to detect gliadins in samples shown as negatives by conventional ELISA. This antibody can be used for detection of gluten in commercial food samples using ELISA (limit of detection 0.24 ppm) and by competitive ELISA using conventional antibodies (limit of detection: 1 ppm) (PMID: 20039674). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |