Mfg Part Number
Ab02078-1.1
Vector Labs Ab02078-1.1 Anti-Mesothelin [C10], Mouse IgG1, kappa (200 μg)
Quick Overview
The original parent antibody SS scFv was generated by intradermally immunizing BALB/c mice with a vector pcD3CAK1-9 which expresses mesothelin under the control of a CMV promoter. Randomized mutations were introduced in the VH and VL CD3 regions to make C10. C10 has S92G-G93S-Y94H mutations in the light chain.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | C10 |
| Synonyms: | MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; SS scFv |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; SPR; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody can recognize and bind human mesothelin. |
| Application Notes: | This antibody is recommended to be used for detection of mesothelin in biological samples. This high affinity anti-mesothelin antibody is derived from its parent antibody SS scFv by incorporating S92G-G93S-Y94H mutations in the light chain. The resulting antibody C10 has a 55 times higher affinity towards mesothelin in an ELISA as compared to its parent antibody. The binding characteristics and affinity of the antibody for mesothelin was determined by surface plasmon resonance (BIAcore assay). C10 binds mesothelin with an affinity of Kd = 0.2 nM. Immunoconjugates with C10 as targeting agent and Pseudomonas exotoxin A (PE38) as immunotoxin can be used for identifying samples containing mesothelin through immunohistochemical assays. The cytotoxic activity of the immunotoxin was also checked on antigen positive A1847 cells (PMID: 10385321, 9435250). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |