Mfg Part Number
Ab02080-10.3
Vector Labs Ab02080-10.3 Anti-Mesothelin [11-25], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with the purified recombinant mesothelin protein.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 11-25 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; FC; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds the human mesothelin protein, which is highly expressed in many human cancers, including the majority of pancreatic adenocarcinomas, ovarian cancers, and mesotheliomas. |
| Application Notes: | It is recommended that this antibody be used in combination with mAb IC14-30 to detect mesothelin with high sensitivity in a capture ELISA. This antibody can also immunoprecipitate recombinant mesothelin. NCI-H226 and NCI-H520 were incubated with anti-MSLN antibodies (11—25) or an isotype-matched control antibody and followed by PE-conjugate anti-mouse IgG. Antigen expression was detected by flow cytometry (Iwahori et al., 2008; PMID: 18394747). 64Cu labeled 11-25 was used in vitro cell binding assay and in vivo positron emission tomography (PET) imaging in the tumor-bearing mice (Kobayashi et al., 2015; PMID: 25883990). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |