Vector Labs Ab02089-1.1 Anti-Dll4 [HMD4-2], Mouse IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: HMD4-2
Synonyms: Delta4; Delta-like protein 4; Delta 4; Drosophila Delta homolog 4
Amount: 200 μg
Application Codes Clone: Blocking; IHC; FC; functional studies
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: HMD4-2 recognizes murine Dll4, one of the five major ligands of the Notch signalling pathway. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumours, and pancreatic, bladder and colonic cancer.
Application Notes: This antibody can be used to investigate the role of the signalling pathways related to the Notch receptors and its ligands. The antibody blocks Notch1-Fc and Notch4-Fc binding to mouse Dll4-expressing cells in a dose-dependent manner and does not bind to cells expressing other mouse Notch ligands, such as Dll1, Jagged1, or Jagged2 (Sekine et al., 2009; 19181931). HMD4-2 was demonstrated as an effective infibitor able to neutralize the Dll4-dependent Notch signalling (Sekine et al., 2009; 19181931). It was used to block Dll4-mediated Notch signaling in LDL-receptor–deficient (Ldlr−/−) mice fed a high-fat, high-cholesterol diet, a model that produces atherosclerosis and metabolic disturbances resembling those seen in the cardiometabolic syndrome in order to test the hypothesis in vivo that Dll4 contributes to the pathogenesis of the cardiometabolic syndrome (Fukuda et al., 2012; pmid: 22699504). This study shown that the Dll4 blockage inhibits atherosclerosis and metabolic disorders (Fukuda et al., 2012; pmid: 22699504). This clone was also utilized in flow cytometry to analyse the expression of Dll4 on splenocytes, dendritic cells and macrophages (Moriyama et al., 2008; pmid: 18381350; Sekine et al., 2009; 19181931). Further, it was also used in the immunohistochemistry to stain frozen murine spleen samples (Sekine et al., 2009; 19181931). It was also used as a part of a control mixture in the functional studies on the maintenance of CD8- dendritic cells (Sekine et al., 2009; 19181931).

Product Class:	Purified
Clone ID:	HMD4-2
Synonyms:	Delta4; Delta-like protein 4; Delta 4; Drosophila Delta homolog 4
Amount:	200 μg
Application Codes Clone:	Blocking; IHC; FC; functional studies
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	HMD4-2 recognizes murine Dll4, one of the five major ligands of the Notch signalling pathway. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumours, and pancreatic, bladder and colonic cancer.
Application Notes:	This antibody can be used to investigate the role of the signalling pathways related to the Notch receptors and its ligands. The antibody blocks Notch1-Fc and Notch4-Fc binding to mouse Dll4-expressing cells in a dose-dependent manner and does not bind to cells expressing other mouse Notch ligands, such as Dll1, Jagged1, or Jagged2 (Sekine et al., 2009; 19181931). HMD4-2 was demonstrated as an effective infibitor able to neutralize the Dll4-dependent Notch signalling (Sekine et al., 2009; 19181931). It was used to block Dll4-mediated Notch signaling in LDL-receptor–deficient (Ldlr−/−) mice fed a high-fat, high-cholesterol diet, a model that produces atherosclerosis and metabolic disturbances resembling those seen in the cardiometabolic syndrome in order to test the hypothesis in vivo that Dll4 contributes to the pathogenesis of the cardiometabolic syndrome (Fukuda et al., 2012; pmid: 22699504). This study shown that the Dll4 blockage inhibits atherosclerosis and metabolic disorders (Fukuda et al., 2012; pmid: 22699504). This clone was also utilized in flow cytometry to analyse the expression of Dll4 on splenocytes, dendritic cells and macrophages (Moriyama et al., 2008; pmid: 18381350; Sekine et al., 2009; 19181931). Further, it was also used in the immunohistochemistry to stain frozen murine spleen samples (Sekine et al., 2009; 19181931). It was also used as a part of a control mixture in the functional studies on the maintenance of CD8- dendritic cells (Sekine et al., 2009; 19181931).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews