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Mfg Part Number

Ab02112-8.4

Vector Labs Ab02112-8.4 Anti-Kv4.2 K+ Channel Ectodomain [K57/1], Rat IgG2b, Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

This antibody was raised by immunising BALB/c mice with a synthetic peptide corresponding to the extracellular S1-S2 loop (amino acids 209-225) of human Kv4.2, where the third Ser was substituted in place of a Cys for peptide conjugation purposes.

$517.40

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Description

Product Class: Purified
Clone ID: K57/1
Heavy Chain Modification: Fc Silent™
Synonyms: K57/1R; Potassium voltage-gated channel subfamily D member 2; Voltage-gated potassium channel subunit Kv4.2; Kcnd2; Kiaa1044; MNCb-7013; RK5; Shal1
Amount: 200 μg
Application Codes Clone: IB; IHC; IP
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the ectodomain of the Kv4.2 potassium channel. It does not react with Kv4.3 and Kv4.1.
Application Notes: This antibody has been used in immunoblot analysis of crude membranes from rat and mouse brain (Andrews et al, 2019; PMID:30667360), as well as crude C. elegans extracts (Andrews et al, 2019; PMID:30667360) and lysates of COS-1 cells transfected to express Kv4.2 (Shibata et al, 2003). It has also been used in immunohistochemical analysis of adult rat and mouse brain sections (Sack et al, 2013; Andrews et al, 2019; PMID:30667360), and immunofluorescence analysis of surface Kv4.2 expression by Kv4.2-transfected COS-1 cells (Shibata et al, 2003). Using ELISA, pre-incubation with soluble Kv4.2 peptide has been shown to block the binding of this antibody to its antigen (Sack et al, 2013). This antibody, when labelled with gold, has been used in electron microscopy to assess endogenous Kv4.2 expression in rat hippocampal sections (Kim et al, 2007).

Product Class:	Purified
Clone ID:	K57/1
Heavy Chain Modification:	Fc Silent™
Synonyms:	K57/1R; Potassium voltage-gated channel subfamily D member 2; Voltage-gated potassium channel subunit Kv4.2; Kcnd2; Kiaa1044; MNCb-7013; RK5; Shal1
Amount:	200 μg
Application Codes Clone:	IB; IHC; IP
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the ectodomain of the Kv4.2 potassium channel. It does not react with Kv4.3 and Kv4.1.
Application Notes:	This antibody has been used in immunoblot analysis of crude membranes from rat and mouse brain (Andrews et al, 2019; PMID:30667360), as well as crude C. elegans extracts (Andrews et al, 2019; PMID:30667360) and lysates of COS-1 cells transfected to express Kv4.2 (Shibata et al, 2003). It has also been used in immunohistochemical analysis of adult rat and mouse brain sections (Sack et al, 2013; Andrews et al, 2019; PMID:30667360), and immunofluorescence analysis of surface Kv4.2 expression by Kv4.2-transfected COS-1 cells (Shibata et al, 2003). Using ELISA, pre-incubation with soluble Kv4.2 peptide has been shown to block the binding of this antibody to its antigen (Sack et al, 2013). This antibody, when labelled with gold, has been used in electron microscopy to assess endogenous Kv4.2 expression in rat hippocampal sections (Kim et al, 2007).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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