Mfg Part Number
Ab02137-8.4
Vector Labs Ab02137-8.4 Anti-Doc2b [N150/21.3], Rat IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunising BALB/c mice with a fusion protein corresponding to amino acids 1-216 (N-terminus) of rat Doc2b.
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| Product Class: | Purified |
|---|---|
| Clone ID: | N150/21.3 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | N150/21.3R; N150/21; Double C2-like domain-containing protein beta; Doc2-beta |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the Doc2b protein and it does not show any cross reactivity with the Doc2a protein. Doc2b is a calcium sensor which positively regulates SNARE-dependent fusion of vesicles with membranes. |
| Application Notes: | This antibody is recommended for the detection and analysis of Doc2b by western blot and immunohistochemistry. For instance, the mouse version of this antibody was used to detect Doc2b by western blot in extracts of RBM and COS cells transiently transfected with GFP-tagged Doc2b or untagged Kv2.1 plasmids. Besides western blot it was also used to detect Doc2b in adult rat hippocampus using immunohistochemistry. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |