Mfg Part Number
Ab02175-10.3
Vector Labs Ab02175-10.3 Anti-NSD3 [N348/82], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 21-251 (N-terminus) of human NSD3
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| Product Class: | Purified |
|---|---|
| Clone ID: | N348/82 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | N348/82R; Histone-lysine N-methyltransferase NSD3; Nuclear SET domain-containing protein 3; Protein whistle; WHSC1-like 1 isoform 9 with methyltransferase activity to lysine; Wolf-Hirschhorn syndrome candidate 1-like protein 1; WHSC1-like protein 1 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; ICC; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for NSD3 epitope: amino acids 21-251 (N-terminus) of human NSD3. NSD3 preferentially dimethylates 'Lys-4' and 'Lys-27' of histone H3 forming H3K2me2 and H3K27me2. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation, while 'Lys-27' is a mark for transcriptional repression. |
| Application Notes: | This antibody is recommended for detection and analysis of NSD3 by immunocytochemistry, immunohistochemistry and western blot. For instance the mouse version of this antibody was used to detect NSD3 by western blot in extracts of COS cells transiently transfected with HA-tagged NSD1, NSD2, NSD3 or untagged Kv2.1 plasmid. Furthermore it was also used to detect NSD3 by immunohistochemistry in adult rat hippocampus. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |