Mfg Part Number
Ab02186-8.4
Vector Labs Ab02186-8.4 Anti-Mortalin/GRP75 [N52A/42.1], Rat IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 551-766 of mouse SALM2. The antibody was off traget.
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| Product Class: | Purified |
|---|---|
| Clone ID: | N52A/42.1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | N52A/42.1R; Stress-70 protein, mitochondrial; Hspa9; 75 kDa glucose-regulated protein; GRP-75; Heat shock 70 kDa protein 9; Mortalin; Peptide-binding protein 74; PBP74; p66 MOT |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IF; IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the mortalin protein. Mortalin is a chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. |
| Application Notes: | The IgG1 version of this antibody was used in a western blot on COS-7 cells and on COS-1 cells (Vincente et al, 2016; PMID:28322444) (Thiffault et al, 2015; PMID:26503721). Immunofluorescense experiments with the IgG1 version of this antibody as positive control were preformed on the mouse brain (Kirmiz et al, 2018; PMID:30012696). The IgG1 version of this antibody has been used for immunoprecipitation on rat brain extracts (Bishop et al, 2018; PMID:29403353). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |