Mfg Part Number
Ab02195-23.0
Vector Labs Ab02195-23.0 Anti-PrP [P-clone], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing mice with amino acid sequence 96-105 of bovine prion protein.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | P-clone |
| Synonyms: | CD230; PRNP; Major prion protein; Major scrapie-associated fibril protein 1 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds amino acid residues 90-120 [ amino acids HG(S,N)QWNKPSKPKTN] or epitope I of the bovine prion protein. This epitope is also present in human PrP as well as in all ungulate PrP sequences, including cattle, deer, elk, kudu, goat, and sheep.This antibody doesnt bind the native disease-associated PrP conformer. It can only recognise the denatured or pretreated PrP, prepared by denaturation and/or proteolysis for peptide-like presentation. |
| Application Notes: | Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative prion disease affecting cattle that is transmissible to humans, manifesting as a variant of Creutzfeldt-Jakob disease (vCJD) likely following the consumption of meat contaminated with BSE prions. This high affinity antibody can be used for detection of infectious prion in a sample. P clone has been used in the development of conformation dependent immunoassay for detection of prion protein in a sample. It can recognise prions from cow, sheep, human, deer, hamster in a western blot assay (PMID:12389035). P clone binds peptide derived from the unstructured region (aa90-105) of bovine PrP (BoPrP) with an affinity of 1pM (PMID: 16962610). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |