Vector Labs Ab02197-10.3 Anti-CD73 [1E9], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 1E9
Heavy Chain Modification: Fc Silent™
Synonyms: NT5E; 5'-NT; 5'-nucleotidase; Ecto-5'-nucleotidase
Amount: 200 μg
Application Codes Clone: Inhibit; IF; IP; IHC; FC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG3
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human CD73, which is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase. This antibody does not show cross reactivity with mouse CD73.
Application Notes: The enzymatic activity of CD73 causes hydrolysis of extracellular nucleotides into membrane permeable nucleosides. This antibody inhibits soluble placental 5'-NT enzymatic activity and recognizes lymphocyte CD73 in indirect immunofluorescence and immunoprecipitation assays. This antibody was also used in the study of cellular distribution of CD73 (PMID: 2137649). It is reported that 1E9 induces vigorous T cell proliferation in the presence of subatomic doses of phorbol myristate and F(ab')2 goat anti-mouse Ig (PMID: 2137649, 2550543). This antibody was also used to study the expression of CD73 in normal mammary gland and breast carcinoma cells using immunohistochemistry (PMID: 1989648). This antibody was also used to detect CD73 in COS cells transfected with CD73 cDNA using flow cytometry (PMID: 7595232). Flow cytometry analysis also showed that anti-CD73 1E9 mAb recognized CD73 on the human breast cancer cell lines LM3 and MDA-MB-231, but failed to detect the murine CD73 variant expressed on isolated mouse mononuclear hematopoietic cells(PMID: 24043904). Bispecific antibody generated using 1E9 and mAb AD2 is reported to inhibit the enzymatic activity of CD73 as well as promotes internalization of CD73.

Product Class:	Purified
Clone ID:	1E9
Heavy Chain Modification:	Fc Silent™
Synonyms:	NT5E; 5'-NT; 5'-nucleotidase; Ecto-5'-nucleotidase
Amount:	200 μg
Application Codes Clone:	Inhibit; IF; IP; IHC; FC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG3
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human CD73, which is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase. This antibody does not show cross reactivity with mouse CD73.
Application Notes:	The enzymatic activity of CD73 causes hydrolysis of extracellular nucleotides into membrane permeable nucleosides. This antibody inhibits soluble placental 5'-NT enzymatic activity and recognizes lymphocyte CD73 in indirect immunofluorescence and immunoprecipitation assays. This antibody was also used in the study of cellular distribution of CD73 (PMID: 2137649). It is reported that 1E9 induces vigorous T cell proliferation in the presence of subatomic doses of phorbol myristate and F(ab')2 goat anti-mouse Ig (PMID: 2137649, 2550543). This antibody was also used to study the expression of CD73 in normal mammary gland and breast carcinoma cells using immunohistochemistry (PMID: 1989648). This antibody was also used to detect CD73 in COS cells transfected with CD73 cDNA using flow cytometry (PMID: 7595232). Flow cytometry analysis also showed that anti-CD73 1E9 mAb recognized CD73 on the human breast cancer cell lines LM3 and MDA-MB-231, but failed to detect the murine CD73 variant expressed on isolated mouse mononuclear hematopoietic cells(PMID: 24043904). Bispecific antibody generated using 1E9 and mAb AD2 is reported to inhibit the enzymatic activity of CD73 as well as promotes internalization of CD73.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews