Vector Labs Ab02200-23.0 Anti-Sporozoite of Eimeria acervulina [6D-12-G10], Rabbit IgG, lambda (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 6D-12-G10
Synonyms: Sporozoite surface antigen; Eimeria acervulina
Amount: 200 μg
Application Codes Clone: WB; IF; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes and binds the apical complex of the sporozoite of Eimeria acervulina, which is a 20-21kDa protein.
Application Notes: Avain coccidiosis is caused by an intracellulae protozoan belonging to the genus Eimeria. This disease is important in aviculture due to its negative effects on production and development of birds. Avian coccidiosis is one of the main causes of growth retardation, poor feed conversion and high mortality in intensive farming of broilers, cage-free pullets and breeders. This mAb recognizes the apical complex of the sporozoite in a western blot assay and ELISA. Immunoelectron microscopic examination revealed that the antibody stained the conoid antigen. In indirect immunofluorescence assay the culture supernatent containing the mAb intensely stained the tip of the E. acervulina sporozoites. It is also reported that this antibody inhibited the invasion of sporozoites in CD8+ T cells in vitro (PMID: 8627507). The scFv antibody was produced at a minimum of 7 mg l−1 and exhibited virtually identical antigen reactivity as the original mAb (Min et al., 2001). mAb 6D-12-G10 shows cross reactivities in immunofluorescence staining at the apical end of sporozoites of C. parvum and C. muris, and merozoites of C. parvum (PMID:15725532). This mAb is also cross reactive with other species like Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp (PMID: 27384177)

Product Class:	Purified
Clone ID:	6D-12-G10
Synonyms:	Sporozoite surface antigen; Eimeria acervulina
Amount:	200 μg
Application Codes Clone:	WB; IF; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes and binds the apical complex of the sporozoite of Eimeria acervulina, which is a 20-21kDa protein.
Application Notes:	Avain coccidiosis is caused by an intracellulae protozoan belonging to the genus Eimeria. This disease is important in aviculture due to its negative effects on production and development of birds. Avian coccidiosis is one of the main causes of growth retardation, poor feed conversion and high mortality in intensive farming of broilers, cage-free pullets and breeders. This mAb recognizes the apical complex of the sporozoite in a western blot assay and ELISA. Immunoelectron microscopic examination revealed that the antibody stained the conoid antigen. In indirect immunofluorescence assay the culture supernatent containing the mAb intensely stained the tip of the E. acervulina sporozoites. It is also reported that this antibody inhibited the invasion of sporozoites in CD8+ T cells in vitro (PMID: 8627507). The scFv antibody was produced at a minimum of 7 mg l−1 and exhibited virtually identical antigen reactivity as the original mAb (Min et al., 2001). mAb 6D-12-G10 shows cross reactivities in immunofluorescence staining at the apical end of sporozoites of C. parvum and C. muris, and merozoites of C. parvum (PMID:15725532). This mAb is also cross reactive with other species like Apicomplexan parasites, including other Eimeria spp., Toxoplasma, Neospora, and Cryptosporidium spp (PMID: 27384177)

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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