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Mfg Part Number

Ab02207-7.1

Vector Labs Ab02207-7.1 Anti-IL-5 [JES1-39D10], Rat IgG2a, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing a male lewis rat with semi-purified recombinant mammalian-expressed IL-5.

$517.40

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Description

Product Class: Purified
Clone ID: JES1-39D10
Synonyms: IL5; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
Amount: 200 μg
Application Codes Clone: Block; ELISA; IHC; FC; immunocytochemistry
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody recognizes and binds human interleukin-5.
Application Notes: This antibody can effectively block IL-5 induced HL-60 histamine content and TF-1 proliferation. 39D10 was also evaluated for its ability to neutralize the ILJ-dependent growth of eosinophilbasophil colonies (i.e., both histamine-positive and histaminenegative Eo-type) in methylcellulose culture. This antibody did not cross-react with mouse IL-5 in indirect ELISA (PMID: 1706953). This antibody was used in immunohistochemical method to study the localisation and phenotype of individual cytokine producing cells in synovial biopsy specimens in rheumatoid arthritis (PMID:7677442). This antibody was also used in immunocytochemical and a flow cytofluorimetric method to detect intracellular IFN-g, IL-4 and IL-5 in T-cell clones, peripheral blood mononuclear cells PBMC and bronchoalveolar lavage fluid BALF cells (PMID: 9134033). The original rat IgG2a antibody binds human IL-5 with a binding affinity of Kd= 53 pM (PMID: 7613159). This antibody can be used in combination with JES1-5A10 for the detection of cytokines in a capture ELISA (PMID: 16827896). Humanized version of this antibody CMX5-2 and CMX5-5 had lower affinities for IL-5 than 39D10, with Kd values that were 1.4 times the Kd for 39D10 (PMID: 8844835).

Product Class:	Purified
Clone ID:	JES1-39D10
Synonyms:	IL5; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF
Amount:	200 μg
Application Codes Clone:	Block; ELISA; IHC; FC; immunocytochemistry
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody recognizes and binds human interleukin-5.
Application Notes:	This antibody can effectively block IL-5 induced HL-60 histamine content and TF-1 proliferation. 39D10 was also evaluated for its ability to neutralize the ILJ-dependent growth of eosinophilbasophil colonies (i.e., both histamine-positive and histaminenegative Eo-type) in methylcellulose culture. This antibody did not cross-react with mouse IL-5 in indirect ELISA (PMID: 1706953). This antibody was used in immunohistochemical method to study the localisation and phenotype of individual cytokine producing cells in synovial biopsy specimens in rheumatoid arthritis (PMID:7677442). This antibody was also used in immunocytochemical and a flow cytofluorimetric method to detect intracellular IFN-g, IL-4 and IL-5 in T-cell clones, peripheral blood mononuclear cells PBMC and bronchoalveolar lavage fluid BALF cells (PMID: 9134033). The original rat IgG2a antibody binds human IL-5 with a binding affinity of Kd= 53 pM (PMID: 7613159). This antibody can be used in combination with JES1-5A10 for the detection of cytokines in a capture ELISA (PMID: 16827896). Humanized version of this antibody CMX5-2 and CMX5-5 had lower affinities for IL-5 than 39D10, with Kd values that were 1.4 times the Kd for 39D10 (PMID: 8844835).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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