Vector Labs Ab02220-10.0 Anti-Orthopoxviruses (incl Ectromelia & Monkeypox) [VACV-5B1], Human IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: VACV-5B1
Synonyms: Orthopoxvirus; Mpox; Monkeypox; Ectromelia virus; ECTV; ECTV-5B1/2G6; EM-5B1/2G6; Mab EX-uUl 5BA 05.11.20
Amount: 200 μg
Application Codes Clone: ELISA; IF; WB; IP; FC; Immunogold electron microscopy
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody recognizes ECTV and some other orthopoxviruses. Its epitope is localized on a tetrapeptide located at the VACV homologue A27 (C-terminal epitope #5 (aa 68–71) (Ahsendorf et al., 2019; pmid: 31146446). Ectromelia virus (ECTV) is a virus of the family Poxviridae and the genus Orthopoxvirus that causes mousepox, a disease of mice. Mousepox causes skin lesions and generalized disease, which can be fatal. Orthopoxvirus has been associated with smallpox, cowpox, horsepox and monkeypox.
Application Notes: This antibody is recommended for the detection and analysis of Orthopox viruses, especially Ectromelia virus (ECTV). It binds to the epitope on the virus which seems not to be engaged with viral cell fusion (Ahsendorf et al., 2019; pmid: 31146446). VACV-5B1 (5B1/2G6) is a non-neutralizing clone that can be utilized for the detection of the analog of A27 protein on Orthopoxviruses (Ahsendorf et al., 2019; pmid: 31146446). This antibody's antigenic site is located in a functionally very important areawithin the C-terminus of the A27 protein; in this hydrophilic region, the two cysteines at positions71 and 72 are responsible for formation of disulfide bonds and, therefore, play an important rolefor a functionally active trimeric A27 structure (Ahsendorf et al., 2019; pmid: 31146446). Mouse IgG2a version of this clone (EM-5B1/2G6) was also used to develop an ELISA capture assay as a capture antibody and was shown to be superior in detecting Orthopox virues in comparison with other monoclonal and polyclonal clones (Johann and Czerny, 1993; pmid: 8122446). It makes it a very good candidate for the development of an anti-orthopox viruse detection assay. In Q3 of 2024, the WHO declared monkeypox a Public Health Emergency of International Concern. This product serves as a powerful tool for related research efforts.

Product Class:	Purified
Clone ID:	VACV-5B1
Synonyms:	Orthopoxvirus; Mpox; Monkeypox; Ectromelia virus; ECTV; ECTV-5B1/2G6; EM-5B1/2G6; Mab EX-uUl 5BA 05.11.20
Amount:	200 μg
Application Codes Clone:	ELISA; IF; WB; IP; FC; Immunogold electron microscopy
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody recognizes ECTV and some other orthopoxviruses. Its epitope is localized on a tetrapeptide located at the VACV homologue A27 (C-terminal epitope #5 (aa 68–71) (Ahsendorf et al., 2019; pmid: 31146446). Ectromelia virus (ECTV) is a virus of the family Poxviridae and the genus Orthopoxvirus that causes mousepox, a disease of mice. Mousepox causes skin lesions and generalized disease, which can be fatal. Orthopoxvirus has been associated with smallpox, cowpox, horsepox and monkeypox.
Application Notes:	This antibody is recommended for the detection and analysis of Orthopox viruses, especially Ectromelia virus (ECTV). It binds to the epitope on the virus which seems not to be engaged with viral cell fusion (Ahsendorf et al., 2019; pmid: 31146446). VACV-5B1 (5B1/2G6) is a non-neutralizing clone that can be utilized for the detection of the analog of A27 protein on Orthopoxviruses (Ahsendorf et al., 2019; pmid: 31146446). This antibody's antigenic site is located in a functionally very important areawithin the C-terminus of the A27 protein; in this hydrophilic region, the two cysteines at positions71 and 72 are responsible for formation of disulfide bonds and, therefore, play an important rolefor a functionally active trimeric A27 structure (Ahsendorf et al., 2019; pmid: 31146446). Mouse IgG2a version of this clone (EM-5B1/2G6) was also used to develop an ELISA capture assay as a capture antibody and was shown to be superior in detecting Orthopox virues in comparison with other monoclonal and polyclonal clones (Johann and Czerny, 1993; pmid: 8122446). It makes it a very good candidate for the development of an anti-orthopox viruse detection assay. In Q3 of 2024, the WHO declared monkeypox a Public Health Emergency of International Concern. This product serves as a powerful tool for related research efforts.

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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