Mfg Part Number
Ab02221-10.3
Vector Labs Ab02221-10.3 Anti-Beta-actin [Fab 19, actin], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original Fab version of this antibody was obtained by recombinant antibody phage display.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | Fab 19, actin |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Actin, cytoplasmic 1; Fab 19; Fab19; Fab 19, actin; Actin, cytoplasmic 1; ACTB; Beta-actin |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted human antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the pointed end of actin filaments (Brawley et al, 2010; pmid: 21103060). Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed: 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. |
| Application Notes: | Immunofluorescence was done with this antibody on actin filaments. In this experiment the antibodies bound to the pointed end of actin (Brawley et al, 2010; pmid: 21103060). ELISA was also preformed with this antibody on actin filaments. This was to confirm the binding of the antibody to actin filaments (Brawley et al, 2010; pmid: 21103060). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |