Vector Labs Ab02223-10.3 Anti-P-selectin [PB1.3], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: PB1.3
Heavy Chain Modification: Fc Silent™
Synonyms: CD62P; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; MAb PB1.3
Amount: 200 μg
Application Codes Clone: Blocking; ELISA; WB; FC; IHC; functional assays
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: PB1.3 recognizes human P-selectin. It reacts with human recombinant P-selectin and purified P-selectin, as well as with P-selectin on thrombin-activated platelets. In contrast, it does not cross-react with recombinant E- or L-selectin. P-selectin is a Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes.
Application Notes: This anti-P-selectin antibody was demonstrated to block the P-selectin-mediated adhesion of human platelets (activated by thrombin) to human neutrophils as well as to inhibit the adhesion to human neutrophils of thrombin-activated rat and rabbit platelets (Mulligan et al., 1992; pmid: 1383277). Furthermore, the administration of PB1.3 in lab animals led to the decrease of lung myeloperoxidase levels, reduced accumulation of neutrophils within the pulmonary interstitial capillaries, diminished adherence of neutrophils to endothelial cells, and greatly reduced hemorrhage (Mulligan et al., 1992; pmid: 1383277). Consequently, this antibody can be recommended for the development of the anti-inflamatory treatments. Additionally, PB1.3 was shown to be able to recognize P-selectin in a platelet preparation via Western blot (Mulligan et al., 1992; pmid: 1383277). Further, this clone was also used to identify the expression of P-selectin on thrombin-activated rat platelets by flow cytometry (Mulligan et al., 1992; pmid: 1383277). Further, the application of the antibody PB1.3 in immunohistochemistry in frozen tissue sections demonstrated that the levels of P-selectin in lung vasculature was rising upon administration of the cobra venom factor (CVF) (Mulligan et al., 1992; pmid: 1383277). Another study demonstrated that the administration of the PB1.3 antibody at the time of reperfusion preserved coronary endothelial nitric oxide release and attenuated myocardial cell necrosis (Lefer et al., 1996; pmid: 8769738). Finally, the same clone was also shown to attenuate lipopolysaccharide-induced acute renal failure in rabbits (Hayashi et al., 2001; pmid: 11287780).

Product Class:	Purified
Clone ID:	PB1.3
Heavy Chain Modification:	Fc Silent™
Synonyms:	CD62P; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; MAb PB1.3
Amount:	200 μg
Application Codes Clone:	Blocking; ELISA; WB; FC; IHC; functional assays
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	PB1.3 recognizes human P-selectin. It reacts with human recombinant P-selectin and purified P-selectin, as well as with P-selectin on thrombin-activated platelets. In contrast, it does not cross-react with recombinant E- or L-selectin. P-selectin is a Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes.
Application Notes:	This anti-P-selectin antibody was demonstrated to block the P-selectin-mediated adhesion of human platelets (activated by thrombin) to human neutrophils as well as to inhibit the adhesion to human neutrophils of thrombin-activated rat and rabbit platelets (Mulligan et al., 1992; pmid: 1383277). Furthermore, the administration of PB1.3 in lab animals led to the decrease of lung myeloperoxidase levels, reduced accumulation of neutrophils within the pulmonary interstitial capillaries, diminished adherence of neutrophils to endothelial cells, and greatly reduced hemorrhage (Mulligan et al., 1992; pmid: 1383277). Consequently, this antibody can be recommended for the development of the anti-inflamatory treatments. Additionally, PB1.3 was shown to be able to recognize P-selectin in a platelet preparation via Western blot (Mulligan et al., 1992; pmid: 1383277). Further, this clone was also used to identify the expression of P-selectin on thrombin-activated rat platelets by flow cytometry (Mulligan et al., 1992; pmid: 1383277). Further, the application of the antibody PB1.3 in immunohistochemistry in frozen tissue sections demonstrated that the levels of P-selectin in lung vasculature was rising upon administration of the cobra venom factor (CVF) (Mulligan et al., 1992; pmid: 1383277). Another study demonstrated that the administration of the PB1.3 antibody at the time of reperfusion preserved coronary endothelial nitric oxide release and attenuated myocardial cell necrosis (Lefer et al., 1996; pmid: 8769738). Finally, the same clone was also shown to attenuate lipopolysaccharide-induced acute renal failure in rabbits (Hayashi et al., 2001; pmid: 11287780).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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