Mfg Part Number
Ab02233-3.0
Vector Labs Ab02233-3.0 Anti-Disialoganglioside 2 [5F11], Mouse IgG2b, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing mice with ganglioside G.sub.D2..sup.9.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 5F11 |
| Synonyms: | GD2 |
| Amount: | 200 μg |
| Application Codes Clone: | IF; ELISA; FC; Therapeutic |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgM |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds the GD2 antigen, but does not cross react with GD3, GT1b, GD1b, GD1a, GM1, GM3 and GM4. . |
| Application Notes: | This antibody is recommended to be used in the detection of GD2 positive tumor cells in the marrow specimens using immunofluorescence staining (PMID: 15136638). It is also recommended for staining of neuroblastoma cells and analysing them using flow cytometry (PMID: 2526848). The anti-GD2 scFv 5F11 recombinantly fused to streptavidin (5F11-scFv-SA) formed a homo-tetramer and showed high GD2 binding. 5F11-scFv-SA allowed inhibition of the binding of anti-5F11 antiidiotypic antibody 1G8 to 5F11 in an enzyme-linked immunosorbent assay (PMID: 15136638). Diphtheria toxins fused to scFv 5F11 demonstrated specific killing of GD2+ tumor cells independent of host immune function (PMID: 11877684). This antibody was also used for the development of bispecific antibody (5F11-scBA) to target disialoganglioside (GD2) positive tumors (PMID: 27471647). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |