Mfg Part Number
Ab02244-10.0
Vector Labs Ab02244-10.0 Anti-Osteocalcin [KTM219], Human IgG1, kappa (200 μg)
Quick Overview
The original antibody was generated by repeatedly immunizing mice with C-terminal hBGP peptide (NH2-FQEAYRRFYGPVCOOH) conjugated with keyhole limpet hemocyanin.
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| Product Class: | Purified |
|---|---|
| Clone ID: | KTM219 |
| Synonyms: | BGP; BGLAP; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody bind the C-terminal end of the human osteocalcin. |
| Application Notes: | This antibody is recommended for detection of low moecular weight peptides by using open sandwich ELISA (OS-ELISA). This antibody detects C-terminal of human osteocalcin in an ELISA. The cloned VH/VL cDNA fragments of this antibody were successful in detecting C-terminal peptides as short as 6 residues in an OS-ELISA. (PMID: 17636882). The VH-MBP and VL-MBP fusion proteins of this antibody were also used in the detection of small biomarker peptide by a micro OS-ELISA integrated into a microfluidic device (PMID: 20024056). The affinity matured version of this antibody O2AG2 was also made using OS-ELISA. It is also available on request (PMID: 20123883). This antibody was also used in the development of a highly sensitive protein based open sandwich immuno-PCR for detection of small biomarkers (PMID: 24025570) |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |