Mfg Part Number
Ab02247-202.1
Vector Labs Ab02247-202.1 Anti-PD-1 [JC053], Dog IgG4 (IgG-D), kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with recombinant canine PD-1 using an exponentially increasing dosing strategy with MPLA as adjuvant.
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| Product Class: | Purified |
|---|---|
| Clone ID: | JC053 |
| Synonyms: | CD279; Programmed death 1 receptor; PD1; PD 1 |
| Amount: | 200 μg |
| Application Codes Clone: | FC, WB, IHC, ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgA |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgA format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds canine PD-1. |
| Application Notes: | This antibody is recommended for detection of canine PD-1. This antibody can detect canine PD-1 in a western blot assay. JC053 successfully detected canine PD-1 expressed on formalin-fixed paraffin-embedded (FFPE) tonsil and lymph node via immunohistochemistry suggesting that this could be useful for histological detection of canine PD-1 and as diagnostic tool in tumor biopsies. JC053 was also used to determine the PD-1 expression on CD4+ and CD8+ T-cells of canine PBMCs stimulated with ConA using flow cytometry. It could also detect recombinant canine PD-1 expression on CHO and CLGL-90 cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-γ level. This antibody did not effectively block PD-1 and did not cause a recovery of T cell function (PMID: 32614928). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |