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Mfg Part Number

Ab02248-201.4

Vector Labs Ab02248-201.4 Anti-PD-L1 [JC071], Dog IgG2 (IgG-B), Fc Silent™, kappa (200 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing BALB/c mice with recombinant canine PD-L1 using an exponentially increasing dosing strategy with MPLA as adjuvant.

$517.40

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Description

Product Class: Purified
Clone ID: JC071
Heavy Chain Modification: Fc Silent™
Synonyms: CD274; Programmed death ligand 1; PDL-1; PDL1
Amount: 200 μg
Application Codes Clone: FC, WB, ELISA, Block
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds canine PD-L1.
Application Notes: This antibody is recommended for detection of canine PD-L1. This antibody successfully detected canine PD-L1 in a western blot assay. JC071 was used to determine the increased PD-1 expression on canine PBMCs stimulated with peptidoglycan or lipopolysaccharide using flow cytometry. JC071 was also able to detect recombinant PD-L1 expressed on DH82 cell line both with and without in vitro activation using IFN-γ in flow cytometry. It also recognized recombinant canine PD-L1 expressed on CHO cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-γ level. JC071 successfully blocked PD-L1 and amplified IFN-γ production in culture by approximately 6-fold. Thus, JC071 alleviated T-cell suppression and led to enhanced functional activity of T cells in culture and have potential application as an immunotherapy reagent for cancer treatment (PMID: 32614928).

Product Class:	Purified
Clone ID:	JC071
Heavy Chain Modification:	Fc Silent™
Synonyms:	CD274; Programmed death ligand 1; PDL-1; PDL1
Amount:	200 μg
Application Codes Clone:	FC, WB, ELISA, Block
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric dog antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds canine PD-L1.
Application Notes:	This antibody is recommended for detection of canine PD-L1. This antibody successfully detected canine PD-L1 in a western blot assay. JC071 was used to determine the increased PD-1 expression on canine PBMCs stimulated with peptidoglycan or lipopolysaccharide using flow cytometry. JC071 was also able to detect recombinant PD-L1 expressed on DH82 cell line both with and without in vitro activation using IFN-γ in flow cytometry. It also recognized recombinant canine PD-L1 expressed on CHO cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-γ level. JC071 successfully blocked PD-L1 and amplified IFN-γ production in culture by approximately 6-fold. Thus, JC071 alleviated T-cell suppression and led to enhanced functional activity of T cells in culture and have potential application as an immunotherapy reagent for cancer treatment (PMID: 32614928).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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