Mfg Part Number
Ab02257-2.0
Vector Labs Ab02257-2.0 Anti-GFP [N86/38.1], Mouse IgG2a, kappa (200 μg)
Quick Overview
This antibody was raised by immunising BALB/c mice with a fusion of protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein.
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| Product Class: | Purified |
|---|---|
| Clone ID: | N86/38.1 |
| Synonyms: | N86/38.1R; Green fluorescent protein |
| Amount: | 200 μg |
| Application Codes Clone: | IF; IP; IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for amino acids 1-238 (full-length) of jellyfish green fluorescent protein. It cross-reacts with cyan and yellow fluorescent protein. It does not cross-react with red fluorescent protein. Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. |
| Application Notes: | Immunofluorescence was preformed with this antibody in the brain of mice. Here it was bound to both yellow and green fluorescent protein (Kumar et al, 2015; PMID:25337655). This antibody was used for immunoprecipitation on Elav-GAL4, 50Y-GAL4, UAS-eGFP, The UAS-GFP and RpL10A fly strains of the genus Drosophila (Thomas et al, 2012; PMID:22792260). Western blotting was preformed with this antibody on samples of Elav-GAL4, 50Y-GAL4, UAS-eGFP, The UAS-GFP and RpL10A fly strains of the genus Drosophila (Thomas et al, 2012; PMID:22792260). Immunohistochemistry was preformed on zebrafish to visualize GFP (Mackenzie and Raible, 2012; PMID:23094043). Immunoprecipitation using this antibody was preformed on the sciatic nerves of rats infected with an adenovirus containing a GFP construct (Chang et al, 2014; PMID:25362471). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |