Mfg Part Number
Ab02281-8.4
Vector Labs Ab02281-8.4 Anti-MMP9 [L51/82R], Rat IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
The original version of this antibody was raised by immunizing a mouse with human MMP-9 residues 107– 445. Then the hybridomas were sequenced by addgene and based on the variable domain sequences new recombinant formats were created.
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| Product Class: | Purified |
|---|---|
| Clone ID: | L51/82R |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; L51/82 |
| Amount: | 200 μg |
| Application Codes Clone: | WB; IHC; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the MMP9 protein. MMP9 could play a role in bone osteoclastic resorption. It cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. |
| Application Notes: | Small bowel and colonic muscularis lysates from C57Bl/6 mice were immunoblotted with this antibody (Moore et al, 2011; PMID:21703213). This antibody was used for immunofluorescence on postnatal brains of 125 Sprague-Dawley rats (Aujla and Huntley, 2014; PMID:24114974). Immunoblot was preformed with this antibody on adult rat whole-hippocampal lysates (Aujla and Huntley, 2014; PMID:24114974). An immunoblot was preformed on HEK293 cells with this antibody (Duellman et al, 2012; PMID:22942228). This antibody was used for immunohistochemistry on HEK293 cells (Duellman et al, 2014; PMID:24627221). A western blot was preformed with this antibody on HEK293 cells (Duellman et al, 2014; PMID:24627221). Mice brain sections were used for immunohistochemistry with this antibody (Kuo et al, 2020; PMID:32926126). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |