Mfg Part Number
Ab02319-10.3
Vector Labs Ab02319-10.3 Anti-Kv1.5 K+ channel [K7/45R], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised by immunizing BALB/c mice with a fusion protein of amino acids 542-602 of rat Kv1.5.
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | K7/45R |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Potassium voltage-gated channel subfamily A member 5; Kcna5; RCK7; RK4; Voltage-gated potassium channel subunit Kv1.5; K7/45 |
| Amount: | 200 μg |
| Application Codes Clone: | IF; IHC; ELISA; IP; functional assays; WB |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for Kv1.5. This antibody recognized a single protein band at 70 kDa on western blot of lysates from Kv1.5-transfected COS cells. No band of equivalent size is observed from rat brain samples and Kv1.5-untransfected COS cells Kv1.5 is a voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. |
| Application Notes: | COS-1 cells were used for immunofluorescence with this antibody (Bekele-Arcuri et al, 1996; PMID:8938716). Immunohistochemistry was preformed on rat brains using this antibody (Bekele-Arcuri et al, 1996; PMID:8938716). GST fusion proteins were used for an ELISA with this antibody (Bekele-Arcuri et al, 1996; PMID:8938716). Immunofluorescence was preformed on COS-1 cells using this antibody (Murakoshi and Trimmer, 1999; PMID:10024359). Proteins from the atrial myocardium and rat brain tissue was immunoprecipitated using this antibody (Godreau et al, 2002; PMID:12445884). proximity ligation (PLA) assay was preformed with this antibody to detect Kv protein-protein interactions (Kilfoil et al, 2019; PMID:31639389). Immunohistochemistry was preformed with this antibody on 50-um-thick sections of mouse brain (Yamada and Jinno, 2013; PMID:22987820). Splenic DCs from Cd11c-Cre-GFP mice were immunoblotted with this antibody (Dong et al, 2019; PMID:31028120). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |