Mfg Part Number
Ab02323-8.4
Vector Labs Ab02323-8.4 Anti-KCNT1/Slo2.2/Slack K+ channel [N3/26R], Rat IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
This antibody was raised against a fusion protein of amino acids 1168-1237 of rat Slo2.2.
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| Product Class: | Purified |
|---|---|
| Clone ID: | N3/26R |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Potassium channel subfamily T member 1; Kcnt1; Sequence like a calcium-activated potassium channel subunit; N3/26 |
| Amount: | 200 μg |
| Application Codes Clone: | IP; WB; IHC; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2a |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for Slo2.2. Slo2.2 is an outwardly rectifying potassium channel subunit that may coassemble with other Slo-type channel subunits. Activated by high intracellular sodium or chloride levels. Activated upon stimulation of G-protein coupled receptors, such as CHRM1 and GRIA1. May be regulated by calcium in the absence of sodium ions (in vitro). |
| Application Notes: | Mouse membrane proteins from whole brain, cerebellum, cortex and spinal cord were immunoprecipitated using this antibody (Martinez-Espinosa et al, 2015; PMID:26559620). Immunoblotting was preformed on mouse membrane proteins from whole brain, cerebellum, cortex and spinal cord using this antibody (Martinez-Espinosa et al, 2015; PMID:26559620). Membrane vesicles from mouse fore- and midbrain were immunoprecipitated using this antibody (Rizzi et al, 2015; PMID:29124216). This antibody was used for immunoblotting on membrane vesicles from mouse fore- and midbrain (Rizzi et al, 2015; PMID:29124216). immunofluorescence was preformed on membrane vesicles from mouse fore- and midbrain using this antibody (Rizzi et al, 2015; PMID:29124216). Untransfected HEK cell lysates or HEK cell lysates transfected with either pcDNA3-Slo2.1 or pcDNA3-Slo2.2 or 20 lg of purified synaptic plasma membrane vesicles were immunoblotted with this antibody (Rizzi et al, 2016; PMID:26587966). C57BL/6J mouse brain sections were used for immunohistochemistry using this antibody (Rizzi et al, 2016; PMID:26587966). The expression of Slack and Slick in rats was assessed for cultured VANs, vestibular epithelium sections and for vestibular ganglion sections. This was done using immunohistochemistry with this antibody (Cervantes et al, 2013; PMID:23466807). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |