Mfg Part Number
Ab02330-10.3
Vector Labs Ab02330-10.3 Anti-CD24 [G7], Human IgG1, Fc Silent™, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with a CD24 core region polypeptide and hemocyanin KLH conjugate (CD24-KLH) as immunogen.
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| Product Class: | Purified |
|---|---|
| Clone ID: | G7 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen |
| Amount: | 200 μg |
| Application Codes Clone: | WB, ELISA, FC, IHC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds human CD24. |
| Application Notes: | This antibody showed specific binding affinity to recombinant human CD24 but not to unrelated recombinant Fc in a western blot. The binding specifity of G7 towards CD24 was also confirmed with competitive ELISA. Flow cytometric assay was carried out to assess the affinities of G7 to native CD24 expressed on Huh7 and HT29 cells with HCT116 cells as control. G7mAb demonstrated significant binding to both Huh7 and HT29 cells with rates 52.9% and 41.3% respectively. This antibody was also used in immunohistochemical staining of tumor samples from Huh7 xenograft nude mice (PMID: 26255089). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |