Vector Labs Ab02333-10.3 Anti-CD4 [OKT4A], Human IgG1, Fc Silent™, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: OKT4A
Heavy Chain Modification: Fc Silent™
Synonyms: T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3
Amount: 200 μg
Application Codes Clone: FC; IF; IHC; functional assay; treatment;
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2a
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds a polymorphic OKT4 epitope on the CD4 molecule. The polymorphism results due to a cytosine-to-thymidine transition occurring at nucleotide position 868, which causes a tryptophan residue to be replaced with an arginine residue at position 240. Persons lacking reactivity with anti-CD4 antibody OKT4 are reactive with OKT4A as well as other mAb specific for CD4. This polymorphic epitope of OKT4 is found in white, black, and Japanese populations. CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, it functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins.
Application Notes: This antibody was used for flow cytometry on human peripheral blood mononuclear cells (Bach et al, 1981; pmid:6790623). This antibody was used in a complement- (C) mediated lysis of T cells (Bach et al, 1981; pmid:6790623). A humanized version of this antibody was used for flow cytometry on Human PBM. This was to test the binding affinity of the newly humanized antibody (Pulito et al, 1996; pmid:8609403). Regions of adult human brain tissue were obtained from a 31-year-old man at autopsy was infected with HIV and then immunostained with this antibody (Jordan et al ,1990; pmid:1702842). The effect of this antibody on immunosuppression in transplant patients was tested. The experiment was preformed in cynomolgus monkeys (Mourad et al, 1998; pmid:9521196). Immunoperoxidase was preformed with this antibody on frozen sections of lymph nodes and sequentially obtained allograft biopsies. The tissues used were obtained from cynomolgus monkeys (Mourad et al, 1998; pmid:9521196). The infection of HIV-1 was determined after binding to this antibody (Zeira et al, 1991; pmid:2034680). MT-4, U-937, and PBL cells were immunostained with this antibody (Schols et al, 1988; pmid:2566170). This antibody was used for treatment of chronic plaque psoriasis (Gottlieb et al, 2000; pmid:11004613).

Product Class:	Purified
Clone ID:	OKT4A
Heavy Chain Modification:	Fc Silent™
Synonyms:	T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3
Amount:	200 μg
Application Codes Clone:	FC; IF; IHC; functional assay; treatment;
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2a
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds a polymorphic OKT4 epitope on the CD4 molecule. The polymorphism results due to a cytosine-to-thymidine transition occurring at nucleotide position 868, which causes a tryptophan residue to be replaced with an arginine residue at position 240. Persons lacking reactivity with anti-CD4 antibody OKT4 are reactive with OKT4A as well as other mAb specific for CD4. This polymorphic epitope of OKT4 is found in white, black, and Japanese populations. CD4 is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, it functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins.
Application Notes:	This antibody was used for flow cytometry on human peripheral blood mononuclear cells (Bach et al, 1981; pmid:6790623). This antibody was used in a complement- (C) mediated lysis of T cells (Bach et al, 1981; pmid:6790623). A humanized version of this antibody was used for flow cytometry on Human PBM. This was to test the binding affinity of the newly humanized antibody (Pulito et al, 1996; pmid:8609403). Regions of adult human brain tissue were obtained from a 31-year-old man at autopsy was infected with HIV and then immunostained with this antibody (Jordan et al ,1990; pmid:1702842). The effect of this antibody on immunosuppression in transplant patients was tested. The experiment was preformed in cynomolgus monkeys (Mourad et al, 1998; pmid:9521196). Immunoperoxidase was preformed with this antibody on frozen sections of lymph nodes and sequentially obtained allograft biopsies. The tissues used were obtained from cynomolgus monkeys (Mourad et al, 1998; pmid:9521196). The infection of HIV-1 was determined after binding to this antibody (Zeira et al, 1991; pmid:2034680). MT-4, U-937, and PBL cells were immunostained with this antibody (Schols et al, 1988; pmid:2566170). This antibody was used for treatment of chronic plaque psoriasis (Gottlieb et al, 2000; pmid:11004613).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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