Vector Labs Ab02339-23.0 Anti-Phosphovimentin [4A4], Rabbit IgG, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 4A4
Synonyms: MPV55; HPV55; phosphorylated vimentin; vimentin phosphoSer55
Amount: 200 μg
Application Codes Clone: ELISA, WB, IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2b
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody specifically binds mouse and human vimentin phosphorylated at serine 55 by cdc2 kinase . This antibody is incapable of binding non-phosphorylated vimentin or with vimentin phosphorylated by other kinases.
Application Notes: This antibody was developed to investigate the role of intermediate filament (IF) protein phosphorylation by cdc2 kinase during mitosis. The binding specificity of 4A4 towards serine 55 phosphorylated vimentin was determined by ELISA. Western blotting indicated that this antibody reacted with vimentin phosphorylated by cdc2 kinase but not with non-phosphorylated vimentin or with vimentin phosphorylated by other kinases such as CAMP-dependent protein kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase 11. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and dephosphorylated in cytokinesis (PMID: 7983050). This antibody was also used in the visualization of mitotic radial glial lineage cells in the developing rat brain which revealed different patterns of gliogenesis in the neocortex and cerebellum (PMID: 9633804). This antibody has been commonly used for the visualization and study of phosphorylation of vimentin during mitosis (PMID: 8601602, 8992382, 9565595).

Product Class:	Purified
Clone ID:	4A4
Synonyms:	MPV55; HPV55; phosphorylated vimentin; vimentin phosphoSer55
Amount:	200 μg
Application Codes Clone:	ELISA, WB, IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2b
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody specifically binds mouse and human vimentin phosphorylated at serine 55 by cdc2 kinase . This antibody is incapable of binding non-phosphorylated vimentin or with vimentin phosphorylated by other kinases.
Application Notes:	This antibody was developed to investigate the role of intermediate filament (IF) protein phosphorylation by cdc2 kinase during mitosis. The binding specificity of 4A4 towards serine 55 phosphorylated vimentin was determined by ELISA. Western blotting indicated that this antibody reacted with vimentin phosphorylated by cdc2 kinase but not with non-phosphorylated vimentin or with vimentin phosphorylated by other kinases such as CAMP-dependent protein kinase, protein kinase C, or Ca2+-calmodulin-dependent protein kinase 11. Immunofluorescence and immunoelectron microscopy showed that vimentin Ser55 residues distributed in the entire cytoplasmic vimentin filament system are phosphorylated when the cells enter mitosis and dephosphorylated in cytokinesis (PMID: 7983050). This antibody was also used in the visualization of mitotic radial glial lineage cells in the developing rat brain which revealed different patterns of gliogenesis in the neocortex and cerebellum (PMID: 9633804). This antibody has been commonly used for the visualization and study of phosphorylation of vimentin during mitosis (PMID: 8601602, 8992382, 9565595).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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