Mfg Part Number
Ab02341-23.0
Vector Labs Ab02341-23.0 Anti-SEB [14G8], Rabbit IgG, kappa (200 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with full-length staphylococcal enterotoxin B (SEB).
$517.40
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| Product Class: | Purified |
|---|---|
| Clone ID: | 14G8 |
| Synonyms: | Staphylococcal enterotoxin B; Enterotoxin type B; entB |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA, WB, Inhibit |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds a conformational epitope near the C terminal end of the Staphylococcal enterotoxin B. This antibody is suspected to interact with residues 135-R, 137-F, 186-Y, 188-K, 231-E, 233-Y, and 235, 236-T. |
| Application Notes: | This antibody in combination with 6D3 and 20B1 can be used to detect Staphylococcal enterotoxin B (SEB) in a sample using capture ELISA. This antibody is capable of detecting SEB in a western blot assay but not staphylococcal enterotoxin A (SEA) or toxic shock syndrome toxin 1 (TSST-1). 14G8 demonstrated comparable level of inhibition of SEB incuded T cell-proliferation in human T-cells from a normal donor. 14G8 also caused inhibition of cytokine induction in treated T-cells as they produced less IFN-gamma and IL-2. It was reported that 14G8 alone did not provide any protection against SEB induced lethal shock (SEBILS) in BALB/c mice and HLA-DR3 transgenic mouse model. However, when used in combination with mAb 6D3 or 20B1 it showed better results (PMID: 21233204). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |