Vector Labs Ab02343-1.1 Anti-SEB [20B1], Mouse IgG1, kappa (200 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 20B1
Synonyms: Staphylococcal enterotoxin B; Enterotoxin type B; entB
Amount: 200 μg
Application Codes Clone: ELISA, WB, Inhibit, NTRL
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody binds a conformational epitope near the C terminal end of the Staphylococcal enterotoxin B. It is suspected that this antibody reacts with 135-R, 137-F, 186-Y, 235- and 236-T of the enterotoxin. This antibody also cross reacts with superantigen SEC-1.
Application Notes: This antibody in combination with 6D3 and 14G8 can be used to detect Staphylococcal enterotoxin B (SEB) in a sample using capture ELISA. This antibody is capable of detecting SEB in a western blot assay but not staphylococcal enterotoxin A (SEA) or toxic shock syndrome toxin 1 (TSST-1). 20B1 demonstrated comparable level of inhibition of SEB incuded T cell-proliferation in human T-cells from a normal donor. 20B1 also caused inhibition of cytokine induction in treated T-cells as they produced less IFN-gamma and IL-2. It was reported that monotherapy with 20B1 alone is capable of neutralizing SEB and provided protection against SEB induced lethal shock (SEBILS) in BALB/c mice and HLA-DR3 transgenic mouse model. It was also recommended that combination with mAb 14G8 or 6D3 enhanced protection and showed better results (PMID: 21233204). In vivo studies indicated that the IgG2a isotype variant of 20B1 exhibited significantly greater protection than IgG1 or IgG2b in murine SEB intoxication and S. aureus sepsis models (PMID: 24917594). ELISA assays confirmed binding of mAb 20B1 to SEC-1, and the binding affinity obtained from BLI analysis was 2.5 +/- 0.45 nM. Superantigens SEC-2, SEC-3 and streptococcal superantigen A (SSA) did not bind mAb 20B1 (PMID: 25572397).

Product Class:	Purified
Clone ID:	20B1
Synonyms:	Staphylococcal enterotoxin B; Enterotoxin type B; entB
Amount:	200 μg
Application Codes Clone:	ELISA, WB, Inhibit, NTRL
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody binds a conformational epitope near the C terminal end of the Staphylococcal enterotoxin B. It is suspected that this antibody reacts with 135-R, 137-F, 186-Y, 235- and 236-T of the enterotoxin. This antibody also cross reacts with superantigen SEC-1.
Application Notes:	This antibody in combination with 6D3 and 14G8 can be used to detect Staphylococcal enterotoxin B (SEB) in a sample using capture ELISA. This antibody is capable of detecting SEB in a western blot assay but not staphylococcal enterotoxin A (SEA) or toxic shock syndrome toxin 1 (TSST-1). 20B1 demonstrated comparable level of inhibition of SEB incuded T cell-proliferation in human T-cells from a normal donor. 20B1 also caused inhibition of cytokine induction in treated T-cells as they produced less IFN-gamma and IL-2. It was reported that monotherapy with 20B1 alone is capable of neutralizing SEB and provided protection against SEB induced lethal shock (SEBILS) in BALB/c mice and HLA-DR3 transgenic mouse model. It was also recommended that combination with mAb 14G8 or 6D3 enhanced protection and showed better results (PMID: 21233204). In vivo studies indicated that the IgG2a isotype variant of 20B1 exhibited significantly greater protection than IgG1 or IgG2b in murine SEB intoxication and S. aureus sepsis models (PMID: 24917594). ELISA assays confirmed binding of mAb 20B1 to SEC-1, and the binding affinity obtained from BLI analysis was 2.5 +/- 0.45 nM. Superantigens SEC-2, SEC-3 and streptococcal superantigen A (SSA) did not bind mAb 20B1 (PMID: 25572397).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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