Mfg Part Number
Ab02373-23.0
Vector Labs Ab02373-23.0 Anti-immunoglobulin [6-B5], Rabbit IgG, kappa (200 μg)
Quick Overview
The original mouse IgG1 version of this antibody was raised against ferret immunoglobulins (IgG + IgM).
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| Product Class: | Purified |
|---|---|
| Clone ID: | 6-B5 |
| Synonyms: | B cell immunoglobulin; Ig; ferret immunoglobulin; B5 |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the ferret B cell immunoglobulin. |
| Application Notes: | This antibody is recommended for the detection of ferret B cell immunoglobulin. It was screened for reactivity against ferret peripheral blood mononuclear cells by FCM what demonstrated specificity for CD79B+ B cells (Kirchenbaum and Ross, 2017; pmid:28286781). It is suitable for Western Blot, ELISA and Flow Cytometry applications. Enzyme-linked immunosorbent assay with the murine version of this antibody (ELISA) was used to assess the reactivity against purified ferret Ig and to determine the IgG subclass and concentration of the respective mAb (Kirchenbaum and Ross, 2017; pmid:28286781). Further, a competitive ELISA was performed using unlabeled and biotinylated MαF-Ig mAb to identify overlapping epitope recognition (Kirchenbaum and Ross, 2017; pmid:28286781). To characterize the specificity of the murine version of this antibody a western blot on ferret immunoglobin was preformed (Kirchenbaum and Ross, 2017; pmid:28286781). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |