Mfg Part Number
Ab02382-3.3
Vector Labs Ab02382-3.3 Anti-SARS-CoV-2 and SARS-CoV nucleocapsid [mBG17], Mouse IgG2b, Fc Silent™, kappa (200 μg)
Quick Overview
The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a recombinant SARS-Cov-2 N protein corresponding to AA133-419.
$517.40
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | mBG17 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | NP; nucleoprotein; NC; Protein N; Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 17; SARS-CoV nucleocapsid |
| Amount: | 200 μg |
| Application Codes Clone: | ELISA; WB; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | mBG17 strongly recognizes SARS-CoV-2 N protein and SARS-CoV N protein. To lesser extent, it cross-reacts with the HuNL63 N protein. Western blot analysis with truncated fragments of N protein suggested that its epitope resides in AA381-419 at the C-terminal. |
| Application Notes: | Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS-Cov was confirmed by Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS-Cov was confirmed by ELISA (Terry et al., 2021; pmid: 33714753). This antibody detected SARS-Cov-2 N protein in Western blot exhibiting labeling patterns suggesting a linear epitope (Terry et al., 2021; pmid: 33714753). mBG17 was shown to be very effective in staining SARS-Cov-2 N protein in immunofluorescence in SARS-Cov-2-infected Vero cells, both methanol and paraformaldehyde fixed (Terry et al., 2021; pmid: 33714753). Finally, the original version of mBG17 was demonstrated to be an effective detection antibody in a sandwich ELISA assay suggesting it's potential use in SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |